| 货号 | 28303S |
| 供应商 | CST |
| 背景 | The CMGC super family of proline-directed protein kinases is a highly conserved and ancient group of proteins that phosphorylate a wide array of substrates involved in stress response, growth and differentiation, and cell division cycle. The consensus sequences targeted by MAPK, SAPK/JNK, CDK, CKII, ERK, and GSK all include a serine or threonine phosphorylation site followed by a proline (1-3). Cell Signaling Technology has developed antibodies that specifically bind to phospho-(S/T)P motifs to enable the study of a large proportion of the phospho-proteome and to identify new substrates of the CMGC kinases. |
| 存放说明 | -20C |
| 参考文献 | 1 . Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. 2 . Shah, O.J. et al. (2003) J Biol Chem 278, 16433-42. 3 . Gonzalez, F.A. et al. (1991) J Biol Chem 266, 22159-63. |
The Motif Logo was generated from a PhosphoScan® LC-MS/MS experiment using 1794 nonredundant tryptic peptides derived from mitotic HeLa cells. HeLa cells were treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (60 nM, 24 hr) and immunoprecipitated using PTMScan® Phospho-Ser/Thr-Pro Motif [p(S/T)P] Immunoaffinity Beads. The logo demonstrates the relative frequency of amino acids in a given position around the central modified residue within this data set. | |
This chart shows the underlying motif distribution in a PhosphoScan® LC-MS/MS experiment using 1794 nonredundant tryptic peptides generated from mitotic HeLa cells. HeLa cells were treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (60 nM, 24 hr) and immunoprecipitated using PTMScan® Phospho-Ser/Thr-Pro Motif [p(S/T)P] Immunoaffinity Beads. |
