| 货号 | 91336S |
| 供应商 | CST |
| 背景 | The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine residues that are followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S/T)P and CDKs phosphorylate substrates containing the consensus sequence (S/T)PXR/K (4,5). Some signaling molecules can be regulated by phosphorylation at a specific threonine followed by an arginine or lysine at the +2 position. For example, conventional PKC isozymes phosphorylate substrates containing a serine or threonine with Arg or Lys at the -3, -2 and +2 positions (6,7). Cell Signaling Technology has developed antibodies that bind to phosphorylation sites containing the T(P/X)R motif for discovery and proteome-wide profiling of kinase substrates. |
| 存放说明 | -20C |
| 参考文献 | 1 . Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41. 2 . Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. 3 . Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95. 4 . Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35. 5 . Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93. 6 . Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6. 7 . Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60. |
The Motif Logo was generated from a PhosphoScan® LC-MS/MS experiment using 261 nonredundant tryptic peptides derived from mouse embryo and immunoprecipitated using PTMScan® Phospho-Thr-X-Arg Motif [pT(P/X)R] Immunoaffinity Beads. | |
This chart shows the distribution of underlying phospho-peptide motifs in a PhosphoScan® LC-MS/MS experiment using 261 tryptic peptides generated from mouse embryo and immunoprecipitated using PTMScan® Phospho-Thr-X-Arg Motif [pT(P/X)R] Immunoaffinity Beads. |
