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Human PDGF-AB Quantikine SixPak (6 Plates) (1 PK)

货号: SHD00C 基本售价: 32598.9 元 规格: 1 PACK

产品信息

概述
货号SHD00C
描述The Quantikine PDGF-AB Immunoassay is a 4.5 hour solid phase ELISA designed to measure human PDGF-AB in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PDGF-AB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-AB showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-AB.
别名PDGFAB; PDGF-AB;
全称
Platelet-derived Growth Factor AB
反应种属Human
目标/特异性Natural and recombinant human PDGF-AB
性能
供应商R&D Systems
检测类型Solid Phase Sandwich ELISA
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
Sci Rep, 2016;6(0):29029.
Species: Human
Sample Type: Cell Culture Supernates

Involvement of Rac in thromboxane A2induced human platelet activation: regulation of sCD40 ligand release and PDGFAB secretion.
Authors: Kageyama Y, Doi T, Matsushima-Nishiwaki R, Iida Y, Akamatsu S, Kondo A, Kuroyanagi G, Yamamoto N, Mizutani J, Otsuka T, Tokuda H, Iida H, Kozawa O, Ogura S
Mol Med Rep, 2014;10(1):107-12.
Species: Human
Sample Type: Plasma

Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
Authors: Chou M, Burnouf T, Wang T
PLoS ONE, 2014;9(6):e99145.
Species: Human
Sample Type: Cell Culture Supernates

Effect of platelet lysate on human cells involved in different phases of wound healing.
Authors: Barsotti M, Losi P, Briganti E, Sanguinetti E, Magera A, Al Kayal T, Feriani R, Di Stefano R, Soldani G
PLoS ONE, 2013;8(12):e84753.
Species: Human
Sample Type: Cell Lysate

Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
BMC Cell Biol, 2013;14(0):48.
Species: Human
Sample Type: Cell Lysates

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
ALTEX, 2011;28(4):305-16.
Species: Human
Sample Type: Cell Lysates

Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch.
Authors: Ding W, Knox TR, Tschumper RC
Blood, 2010;116(16):2984-93.
Species: Human
Sample Type: Platelet-Poor Plasma

Oncogenic and angiogenic growth factors accumulate during routine storage of apheresis platelet concentrates.
Authors: Kanter J, Khan SY, Kelher M, Gore L, Silliman CC
Clin. Cancer Res., 2008;14(12):3942-7.
Species: Human
Sample Type: Platelet-Poor Plasma

Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer.
Authors: Mathew P, Thall PF, Wen S, Bucana C, Jones D, Horne E, Oh WK, Morris MJ, Lee YC, Logothetis CJ, Lin SH, Fidler IJ
Br. J. Cancer, 2008;99(9):1426-32.
Species: Human
Sample Type: Plasma

Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma.
Authors: Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K
Int. J. Cancer, 2007;120(3):686-93.
Species: Human
Sample Type: Cell Culture Supernates

TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC.
Authors: Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T
Arterioscler. Thromb. Vasc. Biol., 2007;28(1):135-41.
Species: Porcine
Sample Type: Cell Culture Supernates

Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
Authors: Pegorier S, Wagner LA, Gleich GJ, Pretolani M
J. Immunol., 2006;177(7):4861-9.
Species: Human
Sample Type: Cell Culture Supernates

Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N
Clin. Cancer Res., 2006;12(9):2676-88.
Species: Human
Sample Type: Cell Culture Supernates

Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
J. Cell. Physiol., 2005;203(1):35-43.
Species: Human
Sample Type: Cell Culture Supernates

Experimental study of the effect of platelet-rich plasma on osteogenesis in rabbit.
Authors: Zhang CQ, Yuan T, Zeng BF
Chin. Med. J., 2004;117(12):1853-5.
Species: Rabbit
Sample Type: Plasma

Autologous fibrin glue with growth factors in reconstructive maxillofacial surgery.
Authors: Thorn JJ, Sorensen H, Weis-Fogh U, Andersen M
Int J Oral Maxillofac Surg, 2004;33(1):95-100.
Species: Human
Sample Type: Fibrogen Solution

Smad3 is required for dedifferentiation of retinal pigment epithelium following retinal detachment in mice.
Authors: Saika S, Kono-Saika S, Tanaka T, Yamanaka O, Ohnishi Y, Sato M, Muragaki Y, Ooshima A, Yoo J, Flanders KC, Roberts AB
Lab. Invest., 2004;84(10):1245-58.
Species: Human
Sample Type: Cell Culture Supernates

Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle.
Authors: Bracamonte MP, Rud KS, Owen WG, Miller VM
Am. J. Physiol. Heart Circ. Physiol., 2002;283(3):H853-60.
Species: Porcine
Sample Type: Cell Lysates

Fibrinogen induces chemotactic activity in endothelial cells.
Authors: Seeger FH, Blessing E, Gu L, Bornhold R, Denger S, Kreuzer J
Acta Physiol. Scand., 2002;176(2):109-15.
Species: Porcine
Sample Type: Cell Culture Supernates

Cyclic mechanical stretching modulates secretion pattern of growth factors in human tendon fibroblasts.
Authors: Skutek M, van Griensven M, Zeichen J, Brauer N, Bosch U
Eur. J. Appl. Physiol., 2001;86(1):48-52.
Species: Human
Sample Type: Cell Culture Supernates

Angiogenic balance in human melanoma: expression of VEGF, bFGF, IL-8, PDGF and angiostatin in relation to vascular density of xenografts in vivo.
Authors: Westphal JR, Vant Hullenaar R, Peek R, Willems RW, Crickard K, Crickard U, Askaa J, Clemmensen I, Ruiter DJ, De Waal RM
Int. J. Cancer, 2000;86(6):768-76.
Species: Human
Sample Type: Cell Culture Supernates

Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation.
Authors: Landesberg R, Roy M, Glickman RS
J. Oral Maxillofac. Surg., 2000;58(3):297.
Species: Human
Sample Type: Plasma

Platelet-derived growth factor (PDGF-AB) like immune reactivity in serum and in cerebral spinal fluid following experimental subarachnoid haemorrhage in dogs.
Authors: Vieweg U, Schramm J, Urbach H
Acta Neurochir (Wien), 1999;141(8):861-5.
Species: Canine
Sample Type: Serum

Growth factors and proinflammatory cytokines in the renal involvement of POEMS syndrome.
Authors: Soubrier M, Sauron C, Souweine B, Larroche C, Wechsler B, Guillevin L, Piette JC, Rousset H, Deteix P
Am. J. Kidney Dis., 1999;34(4):633-8.
Species: Human
Sample Type: Serum

Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells.
Authors: Gu JW, Adair TH
Am. J. Physiol., 1997;273(2):H628-33.
Species: Canine
Sample Type: Cell Culture Supernates

生物活性< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
背景The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
运输条件Blue Ice
存放说明4℃
参考文献
  • PDGF Family
  • PDGF Family Ligands
  • Platelets