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Human IL-5 Quantikine SixPak (6 Plates) (1 PK)

货号: S5000B 基本售价: 31934.7 元 规格: 1 PACK

产品信息

概述
货号S5000B
描述The Quantikine Human IL-5 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-5 levels in cell culture supernates, serum, plasma, and urine. It contains Sf 21-expressed recombinant human IL-5 and antibodies raised against the recombinant factor and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-5 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-5.
别名BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I;
全称
Interleukin 5
反应种属Human
目标/特异性Natural and recombinant human IL-5
性能
供应商R&D Systems
检测类型Solid Phase Sandwich ELISA
Entrez Gene IDs
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Anti-interleukin-5 and multiple autoantibodies are associated with human atherosclerotic diseases and serum interleukin-5 levels.
Authors: Ishigami, Tomoaki, Abe, Kaito, Aoki, Ichiro, Minegishi, Shintaro, Ryo, Akihide, Matsunaga, Satoko, Matsuoka, Kazuhiro, Takeda, Hiroyuki, Sawasaki, Tatsuya, Umemura, Satoshi, Endo, Yaeta
FASEB J, 2013;27(9):3437-45.
Species: Human
Sample Type: Serum

Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
J. Allergy Clin. Immunol., 2010;126(5):962.
Species: Human
Sample Type: Tissue Homogenates

Staphylococcus aureus in nasal lavage and biopsy of patients with chronic rhinosinusitis.
Authors: Niederfuhr A, Kirsche H, Deutschle T, Poppert S, Riechelmann H, Wellinghausen N
Allergy, 2008;63(10):1359-67.
Species: Human
Sample Type: nasal lavage

Detection of pancreatic cancer using antibody microarray-based serum protein profiling.
Authors: Ingvarsson J, Wingren C, Carlsson A, Ellmark P, Wahren B, Engstrom G, Harmenberg U, Krogh M, Peterson C, Borrebaeck CA
Proteomics, 2008;8(11):2211-9.
Species: Human
Sample Type: Serum

Effects of atorvastatin added to inhaled corticosteroids on lung function and sputum cell counts in atopic asthma.
Authors: Hothersall EJ, Chaudhuri R, McSharry C, Donnelly I, Lafferty J, McMahon AD, Weir CJ, Meiklejohn J, Sattar N, McInnes I, Wood S, Thomson NC
Thorax, 2008;63(12):1070-5.
Species: Human
Sample Type: Serum

Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
Int. Immunol., 2008;20(10):1321-9.
Species: Human
Sample Type: Cell Culture Supernates

Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
Eur. J. Immunol., 2008;38(4):1012-23.
Species: Human
Sample Type: Cell Culture Supernates

Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
Authors: Lin YL, Liang YC, Chiang BL
J. Leukoc. Biol., 2007;82(6):1473-80.
Species: Human
Sample Type: Cell Culture Supernates

Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
J. Allergy Clin. Immunol., 2007;120(5):1118-25.
Species: Human
Sample Type: BALF

1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
Authors: Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR
Cytokine, 2007;40(2):128-34.
Species: Human
Sample Type: Cell Culture Supernates

Relationship between eosinophilia and levels of chemokines (CCL5 and CCL11) and IL-5 in bronchoalveolar lavage fluid of patients with mustard gas-induced pulmonary fibrosis.
Authors: Emad A, Emad Y
J. Clin. Immunol., 2007;27(6):605-12.
Species: Human
Sample Type: BALF

Systemic responses after bronchial aspirin challenge in sensitive patients with asthma.
Authors: Makowska JS, Grzegorczyk J, Bienkiewicz B, Wozniak M, Kowalski ML
J. Allergy Clin. Immunol., 2007;121(2):348-54.
Species: Human
Sample Type: Serum

A network-based analysis of the late-phase reaction of the skin.
Authors: Benson M, Langston MA, Adner M, Andersson B, Torinssson-Naluai A, Cardell LO
J. Allergy Clin. Immunol., 2006;118(1):220-5.
Species: Human
Sample Type: Cell Culture Supernates

Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.
Authors: Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M
Am. J. Respir. Crit. Care Med., 2006;173(12):1370-6.
Species: Human
Sample Type: Serum

T-lymphocyte activation and the levels of eosinophilic cationic protein and interleukin-5 in asthmatic children with acute exacerbation and effect of glucocorticoid treatment.
Authors: Kocak AK, Bor O, Yildiz B, Erdogan L, Us T
Allergy Asthma Proc, 2006;27(4):371-7.
Species: Human
Sample Type: Serum

Eotaxin-3 and interleukin-5 pleural fluid levels are associated with pleural fluid eosinophilia in post-coronary artery bypass grafting pleural effusions.
Authors: Kalomenidis I, Stathopoulos GT, Barnette R, Guo Y, Peebles RS, Blackwell TS, Light RW
Chest, 2005;127(6):2094-100.
Species: Human
Sample Type: Pleural Effusion

Respiratory syncytial virus and other respiratory viruses during the first 3 months of life promote a local TH2-like response.
Authors: Kristjansson S, Bjarnarson SP, Wennergren G, Palsdottir AH, Arnadottir T, Haraldsson A, Jonsdottir I
J. Allergy Clin. Immunol., 2005;116(4):805-11.
Species: Human
Sample Type: Nasopharyngeal Secretions

Relationships between allergic inflammation and nasal airflow in children with seasonal allergic rhinitis.
Authors: Ciprandi G, Tosca MA, Marseglia GL, Klersy C
Ann. Allergy Asthma Immunol., 2005;94(2):258-61.
Species: Human
Sample Type: nasal lavage

Antifungal immune reactivity in nasal polyposis.
Authors: Pitzurra L, Bellocchio S, Nocentini A, Bonifazi P, Scardazza R, Gallucci L, Stracci F, Simoncelli C, Bistoni F, Romani L
Infect. Immun., 2004;72(12):7275-81.
Species: Human
Sample Type: nasal lavage

Effects of antiasthmatic agents on the functions of peripheral blood monocyte-derived dendritic cells from atopic patients.
Authors: Saeki S, Matsuse H, Kondo Y, Machida I, Kawano T, Tomari S, Obase Y, Fukushima C, Kohno S
J. Allergy Clin. Immunol., 2004;114(3):538-44.
Species: Human
Sample Type: Cell Culture Supernates

Effect of ozone exposure on airway responses to inhaled allergen in asthmatic subjects.
Authors: Chen LL, Tager IB, Peden DB, Christian DL, Ferrando RE, Welch BS, Balmes JR
Chest, 2004;125(6):2328-35.

Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
Authors: Hellman C, Hallden G, Hylander B, Lundahl J
Clin. Exp. Immunol., 2003;131(1):75-81.
Species: Human
Sample Type: Peritoneal Fluid

Relationship between the perception of dyspnoea and airway inflammatory markers.
Authors: Jang AS, Choi IS
Respir Med, 2002;96(3):150-4.
Species: Human
Sample Type: Saliva

T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
J. Allergy Clin. Immunol., 2002;110(4):624-33.
Species: Human
Sample Type: Cell Culture Supernates

Structural features of peptide analogs of human histocompatibility leukocyte antigen class I epitopes that are more potent and immunogenic than wild-type peptide.
Authors: Tangri S, Ishioka GY, Huang X, Sidney J, Southwood S, Fikes J, Sette A
J. Exp. Med., 2001;194(6):833-46.
Species: Human
Sample Type: Cell Culture Supernates

Low levels of interferon-gamma in nasal fluid accompany raised levels of T-helper 2 cytokines in children with ongoing allergic rhinitis.
Authors: Benson M, Strannegard IL, Wennergren G, Strannegard O
Pediatr Allergy Immunol, 2000;11(1):20-8.
Species: Human
Sample Type: nasal lavage

Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
Authors: Sato E, Koyama S
Eur. Respir. J., 2000;16(5):951-8.
Species: Human
Sample Type: Cell Culture Supernates

Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
J. Exp. Med., 2000;192(2):219-26.
Species: Human
Sample Type: Cell Culture Supernates

Airway inflammation in asthma and perennial allergic rhinitis. Relationship with nonspecific bronchial responsiveness and maximal airway narrowing.
Authors: Alvarez MJ, Olaguibel JM, Garcia BE, Rodriquez A, Tabar AI, Urbiola E
Allergy, 2000;55(4):355-62.
Species: Human
Sample Type: Sputum

生物活性< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
背景IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.
运输条件Blue Ice
存放说明4℃
参考文献
  • Common beta Chain Receptor Family
  • Cytokines and Receptors in Allergy and Asthma
  • Cytokines and Receptors in Tfh Cell Differentiation and Function
  • gamma delta T Cells
  • Granulocyte Markers
  • Group 2 ILCs
  • Inflammatory Mediators
  • T Cell Cytokine Signaling
  • Th2 Cells