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Human Osteopontin (OPN) Quantikine SixPak (6 Plates) (1 PK)

货号: SOST00 基本售价: 29971.6 元 规格: 1 PACK

产品信息

概述
货号SOST00
描述The Quantikine Human Osteopontin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Osteopontin in cell culture supernates, plasma, urine, and human milk. It contains NS0-expressed recombinant human Osteopontin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Osteopontin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Osteopontin.
别名BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin;
全称
Secreted Phosphoprotein 1 [BNSP]
反应种属Human
目标/特异性Natural and recombinant human Osteopontin
性能
供应商R&D Systems
检测类型Solid Phase Sandwich ELISA
Entrez Gene IDs
6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

IL-6 trans-signaling is another pathway to upregulate Osteopontin
Cytokine, 2016;90(0):88-95.
Species: Human
Sample Type: Cell culture supernatant

Osteopontin is a multi-faceted pro-tumorigenic driver for central nervous system lymphoma
Oncotarget, 2016;7(22):32156-71.
Species: Human
Sample Type: Cell Culture Supernates

Targeting of the leukemia microenvironment by c(RGDfV) overcomes the resistance to chemotherapy in acute myeloid leukemia in biomimetic polystyrene scaffolds
Oncol Lett, 2016;12(5):3278-3284.
Species: Human
Sample Type: Cell Culture Supernates

Immunomodulation Induced by Stem Cell Mobilization and Harvesting in Healthy Donors: Increased Systemic Osteopontin Levels after Treatment with Granulocyte Colony-Stimulating Factor
Int J Mol Sci, 2016;17(7):.
Species: Human
Sample Type: Plasma

Epigallocatechin-3-Gallate Upregulates miR-221 to Inhibit Osteopontin-Dependent Hepatic Fibrosis
PLoS ONE, 2016;11(12):e0167435.

Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
JCI Insight, 2016;1(20):e90954.
Species: Human
Sample Type: Cell Culture Supernates

Biomarkers of inflammation and axonal degeneration/damage in patients with newly diagnosed multiple sclerosis: contributions of the soluble CD163 CSF/serum ratio to a biomarker panel.
Authors: Stilund M, Gjelstrup M, Petersen T, Moller H, Rasmussen P, Christensen T
PLoS ONE, 2015;10(4):e0119681.
Species: Human
Sample Type: Serum

Prognostic value of secreted phosphoprotein-1 in pleural effusion associated with non-small cell lung cancer.
Authors: Zhang, He, Liu, Hong-bin, Yuan, Dong-mei, Wang, Zhao-fen, Wang, Yun-fen, Song, Yong
BMC Cancer, 2014;14(0):280.
Species: Human
Sample Type: Pleural Fluid

Prediction of cardiovascular events in statin-treated stable coronary patients of the treating to new targets randomized controlled trial by lipid and non-lipid biomarkers.
Authors: Arsenault B, Barter P, DeMicco D, Bao W, Preston G, LaRosa J, Grundy S, Deedwania P, Greten H, Wenger N, Shepherd J, Waters D, Kastelein J
PLoS ONE, 2014;9(12):e114519.
Species: Human
Sample Type: Plasma

Osteopontin is a prognostic biomarker in non-small cell lung cancer.
Authors: Rud A, Boye K, Oijordsbakken M, Lund-Iversen M, Halvorsen A, Solberg S, Berge G, Helland A, Brustugun O, Maelandsmo G
BMC Cancer, 2013;13(0):540.
Species: Human
Sample Type: Serum

Thrombin-cleaved fragments of osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage.
Authors: Yamaguchi Y, Shao Z, Sharif S, Du X, Myles T, Merchant M, Harsh G, Glantz M, Recht L, Morser J, Leung L
J Biol Chem, 2013;288(5):3097-111.
Species: Human
Sample Type: Tissue Homogenates

Prognostic utility of pre-operative circulating osteopontin, carbonic anhydrase IX and CRP in renal cell carcinoma.
Br. J. Cancer, 2012;107(7):1131-7.
Species: Human
Sample Type: Plasma

A multi-analyte assay for the non-invasive detection of bladder cancer.
Authors: Goodison, Steve, Chang, Myron, Dai, Yunfeng, Urquidi, Virginia, Rosser, Charles
PLoS ONE, 2012;7(10):e47469.
Species: Human
Sample Type: Urine

Osteopontin is a prognostic factor for survival of acute myeloid leukemia patients.
Authors: Liersch R, Gerss J, Schliemann C, Bayer M, Schwoppe C, Biermann C, Appelmann I, Kessler T, Lowenberg B, Buchner T, Hiddemann W, Muller-Tidow C, Berdel WE, Mesters R
Blood, 2012;119(22):5215-20.
Species: Human
Sample Type: Plasma

Plasma markers for identifying patients with metastatic melanoma.
Authors: Kluger HM, Hoyt K, Bacchiocchi A, Mayer T, Kirsch J, Kluger Y, Sznol M, Ariyan S, Molinaro A, Halaban R
Clin. Cancer Res., 2011;17(8):2417-25.
Species: Human
Sample Type: Plasma

Serum levels of beta-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma.
Authors: Zekri A, Bahnassy A, Alam El-Din H, Morsy H, Shaarawy S, Moharram N, Daoud S
Oncol Rep, 2011;26(4):825-31.
Species: Human
Sample Type: Serum

An in vivo platform for tumor biomarker assessment.
Authors: Servais EL, Suzuki K, Colovos C
PLoS ONE, 2011;6(10):e26722.
Species: Human
Sample Type: Plasma

Osteopontin as a novel prognostic marker in stable ischaemic heart disease: a 3-year follow-up study.
Authors: Georgiadou P, Iliodromitis EK, Kolokathis F, Varounis C, Gizas V, Mavroidis M, Capetanaki Y, Boudoulas H, Kremastinos DT
Eur. J. Clin. Invest., 2010;0(0):.
Species: Human
Sample Type: Plasma

Changes in osteopontin and in biomarkers of bone turnover during human endotoxemia.
Authors: Grimm G, Vila G, Bieglmayer C
Bone, 2010;47(2):388-91.
Species: Human
Sample Type: Plasma

Circulating levels of osteopontin are closely related to glomerular filtration rate and cardiovascular risk markers in patients with chronic kidney disease.
Authors: Lorenzen J, Kramer R, Kliem V, Bode-Boeger SM, Veldink H, Haller H, Fliser D, Kielstein JT
Eur. J. Clin. Invest., 2010;40(4):294-300.
Species: Human
Sample Type: Plasma

A multi-analyte serum test for the detection of non-small cell lung cancer.
Authors: Farlow EC, Vercillo MS, Coon JS, Basu S, Kim AW, Faber LP, Warren WH, Bonomi P, Liptay MJ, Borgia JA
Br. J. Cancer, 2010;103(8):1221-8.
Species: Human
Sample Type: Serum

Osteopontin is expressed and functional in human eosinophils.
Authors: Puxeddu I, Berkman N, Ribatti D
Allergy, 2010;65(2):168-74.
Species: Human
Sample Type: BALF

Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome.
Authors: Campeau PM, Rafei M, Boivin MN, Sun Y, Grabowski GA, Galipeau J
Blood, 2009;114(15):3181-90.
Species: Human
Sample Type: Cell Culture Supernates

Activation of the osteopontin/matrix metalloproteinase-9 pathway correlates with prostate cancer progression.
Authors: Castellano G, Malaponte G, Mazzarino MC, Figini M, Marchese F, Gangemi P, Travali S, Stivala F, Canevari S, Libra M
Clin. Cancer Res., 2008;14(22):7470-80.
Species: Human
Sample Type: Plasma

Intensive lipid-lowering therapy ameliorates novel calcification markers and GSM score in patients with carotid stenosis.
Authors: Kadoglou NP, Gerasimidis T, Moumtzouoglou A, Kapelouzou A, Sailer N, Fotiadis G, Vitta I, Katinios A, Kougias P, Bandios S, Voliotis K, Karayannacos PE, Liapis CD
Eur J Vasc Endovasc Surg, 2008;35(6):661-8.
Species: Human
Sample Type: Plasma

Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.
Authors: Zdzisinska B, Bojarska-Junak A, Dmoszynska A, Kandefer-Szerszen M
Arch. Immunol. Ther. Exp. (Warsz.), 2008;56(3):207-21.
Species: Human
Sample Type: Cell Culture Supernates

Innate immune responses to TREM-1 activation: overlap, divergence, and positive and negative cross-talk with bacterial lipopolysaccharide.
Authors: Dower K, Ellis DK, Saraf K, Jelinsky SA, Lin LL
J. Immunol., 2008;180(5):3520-34.
Species: Human
Sample Type: Cell Culture Supernates

Abrogation of T cell quiescence characterizes patients at high risk for multiple sclerosis after the initial neurological event.
Authors: Corvol JC, Pelletier D, Henry RG, Caillier SJ, Wang J, Pappas D, Casazza S, Okuda DT, Hauser SL, Oksenberg JR, Baranzini SE
Proc. Natl. Acad. Sci. U.S.A., 2008;105(33):11839-44.
Species: Human
Sample Type: Plasma

The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesis.
Authors: Colla S, Tagliaferri S, Morandi F, Lunghi P, Donofrio G, Martorana D, Mancini C, Lazzaretti M, Mazzera L, Ravanetti L, Bonomini S, Ferrari L, Miranda C, Ladetto M, Neri TM, Neri A, Greco A, Mangoni M, Bonati A, Rizzoli V, Giuliani N
Blood, 2007;110(13):4464-75.
Species: Human
Sample Type: Cell Culture Supernates

Heterozygous disruption of the PTEN promotes intestinal neoplasia in APCmin/+ mouse: roles of osteopontin.
Authors: Shao J, Washington MK, Saxena R, Sheng H
Carcinogenesis, 2007;28(12):2476-83.
Species: Human
Sample Type: Cell Culture Supernates

An integrated clinical-genomics approach identifies a candidate multi-analyte blood test for serous ovarian carcinoma.
Authors: Meinhold-Heerlein I, Bauerschlag D, Zhou Y, Sapinoso LM, Ching K, Frierson H, Brautigam K, Sehouli J, Stickeler E, Konsgen D, Hilpert F, von Kaisenberg CS, Pfisterer J, Bauknecht T, Jonat W, Arnold N, Hampton GM
Clin. Cancer Res., 2007;13(2):458-66.
Species: Human
Sample Type: Serum

Osteopontin expression and serum levels in metastatic uveal melanoma: a pilot study.
Authors: Pe'er J
Invest. Ophthalmol. Vis. Sci., 2006;47(3):802-6.
Species: Human
Sample Type: Serum

生物活性< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
背景Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.
运输条件Blue Ice
存放说明4℃
参考文献
  • Adhesion Molecules in Angiogenesis
  • Adhesion Molecules on VSMC
  • Adipose-derived Stem Cell Markers
  • Cancer Biomarkers
  • Extracellular Matrix Molecules
  • Gametes and Fertilization
  • HIF Transcription Factors
  • Integrins and Associated Molecules
  • Molecules Secreted by VSMC
  • Osteoblast Markers
  • Osteogenesis Markers
  • Renal Markers