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Human CCL3/MIP-1 alpha Quantikine SixPak (6 Plates) (1 PK)

货号: SMA00 基本售价: 29971.6 元 规格: 1 PACK

产品信息

概述
货号SMA00
描述The Quantikine Human MIP-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MIP-1 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human MIP-1 alpha.
别名 C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3;
反应种属Human
目标/特异性Natural and recombinant human MIP-1 alpha
性能
供应商R&D Systems
检测类型Solid Phase Sandwich ELISA
Entrez Gene IDs
6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Functional Differences between IgM and IgD Signaling in Chronic Lymphocytic Leukemia
J Immunol, 2016;0(0):.
Species: Human
Sample Type: Cell Culture Supernates

The phosphoinositide-3-kinase (PI3K)-delta and gamma inhibitor, IPI-145 (Duvelisib), overcomes signals from the PI3K/AKT/S6 pathway and promotes apoptosis in CLL.
Authors: Balakrishnan K, Peluso M, Fu M, Rosin N, Burger J, Wierda W, Keating M, Faia K, OBrien S, Kutok J, Gandhi V
Leukemia, 2015;29(9):1811-22.
Species: Human
Sample Type: Cell Culture Supernates

The Bruton tyrosine kinase inhibitor PCI-32765 thwarts chronic lymphocytic leukemia cell survival and tissue homing in vitro and in vivo.
Authors: Ponader S, Chen SS, Buggy JJ
Blood, 2012;119(5):1182-9.
Species: Human
Sample Type: Plasma

Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
Authors: Johnson TR, McLellan JS, Graham BS
J. Virol., 2012;86(3):1339-47.
Species: Human
Sample Type: Cell Culture Supernates

Alveolar fibrocyte percentage is an independent predictor of poor outcome in patients with acute lung injury.
Authors: Quesnel C, Piednoir P, Gelly J, Nardelli L, Garnier M, Lecon V, Lasocki S, Bouadma L, Philip I, Elbim C, Mentre F, Soler P, Crestani B, Dehoux M
Crit. Care Med., 2012;40(1):21-8.
Species: Human
Sample Type: BALF

CCL3 (MIP-1alpha) plasma levels and the risk for disease progression in chronic lymphocytic leukemia.
Authors: Sivina M, Hartmann E, Kipps TJ, Rassenti L, Krupnik D, Lerner S, LaPushin R, Xiao L, Huang X, Werner L, Neuberg D, Kantarjian H, OBrien S, Wierda WG, Keating MJ, Rosenwald A, Burger JA
Blood, 2011;117(5):1662-9.
Species: Human
Sample Type: Plasma

A phase I trial of bortezomib with temozolomide in patients with advanced melanoma: toxicities, antitumor effects, and modulation of therapeutic targets.
Authors: Su Y, Amiri KI, Horton LW
Clin. Cancer Res., 2010;16(1):348-57.
Species: Human
Sample Type: Serum

Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
Authors: Mabilleau G, Sabokbar A
PLoS ONE, 2009;4(1):e4173.
Species: Human
Sample Type: Cell Culture Supernates

HIV increases markers of cardiovascular risk: results from a randomized, treatment interruption trial.
Authors: Calmy A, Gayet-Ageron A, Montecucco F, Nguyen A, Mach F, Burger F, Ubolyam S, Carr A, Ruxungtham K, Hirschel B, Ananworanich J,
AIDS, 2009;23(8):929-39.
Species: Human
Sample Type: Plasma

Duffy antigen modifies the chemokine response in human endotoxemia.
Authors: Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B
Crit. Care Med., 2008;36(1):159-65.
Species: Human
Sample Type: Plasma

Chronically inflamed human tissues are infiltrated by highly differentiated Th17 lymphocytes.
Authors: Pene J, Chevalier S, Preisser L, Venereau E, Guilleux MH, Ghannam S, Moles JP, Danger Y, Ravon E, Lesaux S, Yssel H, Gascan H
J. Immunol., 2008;180(0):7423.
Species: Human
Sample Type: Cell Culture Supernates

In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
J. Interferon Cytokine Res., 2008;28(5):297-310.
Species: Human
Sample Type: Cell Culture Supernates

Expression of CXC and CC type of chemokines and its receptors in tuberculous and non-tuberculous effusions.
Authors: Pokkali S, Das SD, R L
Cytokine, 2008;41(3):307-14.
Species: Human
Sample Type: Serum

Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
Int. J. Cancer, 2008;122(4):769-76.
Species: Human
Sample Type: Cell Culture Supernates

MMP-12 induces IL-8/CXCL8 secretion through EGFR and ERK1/2 activation in epithelial cells.
Authors: Le Quement C, Guenon I, Gillon JY, Lagente V, Boichot E
Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(6):L1076-84.
Species: Human
Sample Type: Cell Culture Supernates

Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
Proc. Natl. Acad. Sci. U.S.A., 2007;104(7):2319-24.
Species: Human
Sample Type: Cell Culture Supernates

IL-18 is induced and IL-18 receptor alpha plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation.
Authors: Kang MJ, Homer RJ, Gallo A, Lee CG, Crothers KA, Cho SJ, Rochester C, Cain H, Chupp G, Yoon HJ, Elias JA
J. Immunol., 2007;178(3):1948-59.
Species: Human
Sample Type: BALF

Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES.
Authors: Tikhonov I, Deetz CO, Paca R, Berg S, Lukyanenko V, Lim JK, Pauza CD
Int. Immunol., 2006;18(8):1243-51.
Species: Human
Sample Type: Cell Lysates

Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
Cytokine, 2006;36(5):237-44.
Species: Human
Sample Type: Cell Culture Supernates

Interleukin-25-induced chemokines and interleukin-6 release from eosinophils is mediated by p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and nuclear factor-kappaB.
Authors: Wong CK, Cheung PF, Ip WK, Lam CW
Am. J. Respir. Cell Mol. Biol., 2005;33(2):186-94.
Species: Human
Sample Type: Cell Culture Supernates

CCR5 expression and CC chemokine levels in idiopathic pulmonary fibrosis.
Authors: Capelli A, Di Stefano A, Gnemmi I, Donner CF
Eur. Respir. J., 2005;25(4):701-7.
Species: Human
Sample Type: BALF

Concentrations of macrophage inflammatory proteins MIP-1alpha and MIP-1beta and interleukin 8 (il-8) in lyme borreliosis.
Authors: Grygorczuk S, Pancewicz S, Zajkowska J, Kondrusik M, Rwierzbinska R, Hermanowska-Szpakowicz T
Infection, 2004;32(6):350-5.
Species: Human
Sample Type: CSF

Role for cysteinyl leukotrienes in allergen-induced change in circulating dendritic cell number in asthma.
Authors: Parameswaran K, Liang H, Fanat A, Watson R, Snider DP, O'Byrne PM
J. Allergy Clin. Immunol., 2004;114(1):73-9.
Species: Human
Sample Type: Sputum

Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages.
Authors: Emori Y, Ikeda T, Ohashi T, Masuda T, Kurimoto T, Takei M, Kannagi M
J. Gen. Virol., 2004;85(0):2603-13.
Species: Human
Sample Type: Cell Culture Supernates

Up-regulation of XCR1 expression in rheumatoid joints.
Authors: Wang CR, Liu MF, Huang YH, Chen HC
Rheumatology (Oxford), 2004;43(5):569-73.
Species: Human
Sample Type: Serum

Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia.
Authors: Choi ES, Jakubzick C, Carpenter KJ, Kunkel SL, Evanoff H, Martinez FJ, Flaherty KR, Toews GB, Colby TV, Kazerooni EA, Gross BH, Travis WD, Hogaboam CM
Am. J. Respir. Crit. Care Med., 2004;170(5):508-15.
Species: Human
Sample Type: Cell Culture Supernates

Regulation of CCR5 expression and MIP-1alpha production in CD4+ T cells from patients with rheumatoid arthritis.
Authors: Wang CR, Liu MF
Clin. Exp. Immunol., 2003;132(2):371-8.
Species: Human
Sample Type: Cell Culture Supernates

TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion.
Authors: Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M
Eur. J. Immunol., 2002;32(7):2037-45.
Species: Human
Sample Type: Cell Culture Supernates

Analysis of chemokines and chemokine receptor expression in ovarian cancer ascites.
Authors: Milliken D, Scotton C, Raju S, Balkwill F, Wilson J
Clin. Cancer Res., 2002;8(4):1108-14.
Species: Human
Sample Type: Plasma

Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
Authors: Binninger-Schinzel D, Norley S, Adler HS, Oberg HH, Kurth R
Virology, 2002;294(1):209-21.
Species: Human
Sample Type: Cell Culture Supernates

CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor.
Authors: Honczarenko M, Le Y, Glodek AM, Silberstein LE
Blood, 2002;100(7):2321-9.
Species: Human
Sample Type: Cell Culture Supernates

Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
J. Immunol., 2002;169(8):4172-82.
Species: Human
Sample Type: Cell Culture Supernates

Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
Immunity, 2001;14(6):705-14.
Species: Human
Sample Type: Cell Culture Supernates

Distinct hematopoietic support by two human stromal cell lines.
Authors: Loeuillet C, Bernard G, Remy-Martin J, Saas P, Herve P, Douay L, Chalmers D
Exp. Hematol., 2001;29(6):736-45.
Species: Human
Sample Type: Cell Culture Supernates

Chemoattractant factors in breast milk from allergic and nonallergic mothers.
Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
Pediatr. Res., 2000;47(5):592-7.
Species: Human
Sample Type: Serum

Up-regulation of beta-chemokines and down-modulation of CCR5 co-receptors inhibit simian immunodeficiency virus transmission in non-human primates.
Authors: Lehner T, Wang Y, Cranage M, Tao L, Mitchell E, Bravery C, Doyle C, Pratt K, Hall G, Dennis M, Villinger L, Bergmeier L
Immunology, 2000;99(4):569-77.
Species: Primate - Macaca mulatta (Rhesus monkey)
Sample Type: Cell Culture Supernates

Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia.
Authors: Yates SL, Burgess LH, Kocsis-Angle J, Antal JM, Dority MD, Embury PB, Piotrkowski AM, Brunden KR
J. Neurochem., 2000;74(3):1017-25.
Species: Human
Sample Type: Cell Culture Supernates

Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees.
Authors: Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, Van Deventer SJ, van der Poll T
J. Infect. Dis., 1999;179(3):646-52.
Species: Primate - Pan troglodytes (Chimpanzee)
Sample Type: Plasma

Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and beta-chemokine production.
Authors: Gauduin MC, Glickman RL, Ahmad S, Yilma T, Johnson RP
Proc. Natl. Acad. Sci. U.S.A., 1999;96(24):14031-6.
Species: Primate
Sample Type: Cell Culture Supernates

Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques.
Authors: Lehner T, Wang Y, Cranage M, Bergmeier LA, Mitchell E, Tao L, Hall G, Dennis M, Cook N, Brookes R, Klavinskis L, Jones I, Doyle C, Ward R
Nat. Med., 1996;2(7):767-75.
Species: Primate - Macaca mulatta (Rhesus monkey)
Sample Type: Cell Culture Supernates

生物活性< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
背景

MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils.

The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78a and CCL3L1/LD78b, which share 94% amino acid sequence homology. Whereas the human CCL3/LD78a is a single-copy gene, the human CCL3L1/LD78b gene copy number varies within the population. Human CCL3L1/LD78b binds and signals through chemokine receptors CCR1, CCR5. When compared to CCL3/LD78a, CCL3L1/LD78b has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.

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参考文献
  • CC Chemokines and Receptors
  • Chemokines and Receptors
  • Chemokines and Receptors in Allergy and Asthma
  • Chemokines and Receptors in Angiogenesis
  • Group 1 ILCs
  • Infectious Diseases
  • Inflammatory Mediators
  • Macrophage Activation Markers
  • Molecules Secreted by VSMC
  • Multiple Myeloma
  • Neuroinflammation
  • Platelets