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Human GM-CSF Quantikine SixPak (6 Plates) (1 PK)

货号: SGM00 基本售价: 29971.6 元 规格: 1 PACK

产品信息

概述
货号SGM00
描述The Quantikine Human GM-CSF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure GM-CSF levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human GM-CSF and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human GM-CSF accurately. Results obtained using natural human GM-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural GM-CSF.
别名colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; molgramostin; sargramostim;
全称
Granulocyte Macrophage Growth Factor
反应种属Human
目标/特异性Natural and recombinant human GM-CSF
性能
供应商R&D Systems
检测类型Solid Phase Sandwich ELISA
Entrez Gene IDs
1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Association between Placental Lesions, Cytokines and Angiogenic Factors in Pregnant Women with Preeclampsia
Authors: Ingrid C Weel
PLoS ONE, 2016;11(6):e0157584.
Species: Human
Sample Type: Tissue Homogenates

The Novel Receptor C5aR2 Is Required for C5a-Mediated Human Mast Cell Adhesion, Migration, and Proinflammatory Mediator Production.
Authors: Pundir P, MacDonald C, Kulka M
J Immunol, 2015;195(6):2774-87.
Species: Human
Sample Type: Cell Culture Supernates

Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli?
Authors: Spampinato S, Obermeier B, Cotleur A, Love A, Takeshita Y, Sano Y, Kanda T, Ransohoff R
PLoS ONE, 2015;10(7):e0133392.
Species: Human
Sample Type: Cell Culture Supernates

Phase 1b Trial of Biweekly Intravenous Pexa-Vec (JX-594), an Oncolytic and Immunotherapeutic Vaccinia Virus in Colorectal Cancer.
Authors: Park S, Breitbach C, Lee J, Park J, Lim H, Kang W, Moon A, Mun J, Sommermann E, Maruri Avidal L, Patt R, Pelusio A, Burke J, Hwang T, Kirn D, Park Y
Mol Ther, 2015;23(9):1532-40.
Species: Human
Sample Type: Plasma

Autophagy promotes radiation-induced senescence but inhibits bystander effects in human breast cancer cells.
Authors: Huang, Yao-Huei, Yang, Pei-Ming, Chuah, Qiu-Yu, Lee, Yi-Jang, Hsieh, Yi-Fen, Peng, Chih-Wen, Chiu, Shu-Jun
Autophagy, 2014;10(7):1212-28.
Species: Human
Sample Type: Cell Culture Supernates

Prevention of F-actin assembly switches the response to SCF from chemotaxis to degranulation in human mast cells.
Authors: Smrz D, Bandara G, Beaven M, Metcalfe D, Gilfillan A
Eur J Immunol, 2013;43(7):1873-82.
Species: Human
Sample Type: Cell Culture Supernates

Anti-inflammatory effects of methoxyphenolic compounds on human airway cells.
Authors: Houser KR, Johnson DK, Ishmael FT
J Inflamm (Lond), 2012;9(0):6.
Species: Human
Sample Type: Cell Culture Supernates

p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype.
Authors: Freund A, Patil CK, Campisi J
EMBO J., 2011;30(8):1536-48.
Species: Human
Sample Type: Cell Culture Supernates

The implication of aberrant GM-CSF expression in decidual cells in the pathogenesis of preeclampsia.
Authors: Huang SJ, Zenclussen AC, Chen CP, Basar M, Yang H, Arcuri F, Li M, Kocamaz E, Buchwalder L, Rahman M, Kayisli U, Schatz F, Toti P, Lockwood CJ
Am. J. Pathol., 2010;177(5):2472-82.
Species: Human
Sample Type: Tissue Homogenates

Shaping of iNKT cell repertoire after unrelated cord blood transplantation.
Authors: Beziat V, Nguyen S, Exley M, Achour A, Simon T, Chevallier P, Sirvent A, Vigouroux S, Debre P, Rio B, Vieillard V, Lubrano-Berthelier C, Alatrakchi N
Clin. Immunol., 2010;135(3):364-73.
Species: Human
Sample Type: Cell Culture Supernates

HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
J. Exp. Med., 2009;206(1):169-81.
Species: Human
Sample Type: Cell Culture Supernates

HIV increases markers of cardiovascular risk: results from a randomized, treatment interruption trial.
Authors: Calmy A, Gayet-Ageron A, Montecucco F, Nguyen A, Mach F, Burger F, Ubolyam S, Carr A, Ruxungtham K, Hirschel B, Ananworanich J,
AIDS, 2009;23(8):929-39.
Species: Human
Sample Type: Plasma

Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
J. Immunol., 2008;181(11):8053-67.
Species: Human
Sample Type: BALF

Assessment of cervicovaginal cytokine levels following exposure to microbicide Nisin gel in rabbits.
Authors: Aranha CC, Gupta SM, Reddy KV
Cytokine, 2008;43(1):63-70.
Species: Rabbit
Sample Type: cervicovaginal lavage

Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
Exp. Hematol., 2008;36(8):1035-46.
Species: Human
Sample Type: Cell Culture Supernates

Systemic responses after bronchial aspirin challenge in sensitive patients with asthma.
Authors: Makowska JS, Grzegorczyk J, Bienkiewicz B, Wozniak M, Kowalski ML
J. Allergy Clin. Immunol., 2007;121(2):348-54.
Species: Human
Sample Type: Serum

Transmission of Mycobacterium tuberculosis undetected by tuberculin skin testing.
Authors: Anderson ST, Williams AJ, Brown JR, Newton SM, Simsova M, Nicol MP, Sebo P, Levin M, Wilkinson RJ, Wilkinson KA
Am. J. Respir. Crit. Care Med., 2006;173(9):1038-42.
Species: Human
Sample Type: Cell Culture Supernates

Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities.
Authors: Pang L, Nie M, Corbett L, Sutcliffe A, Knox AJ
J. Immunol., 2006;176(6):3788-95.
Species: Human
Sample Type: Cell Culture Supernates

cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis.
Authors: Vassina EM, Yousefi S, Simon D, Zwicky C, Conus S, Simon HU
Eur. J. Immunol., 2006;36(7):1975-84.
Species: Human
Sample Type: Serum

Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
Int. J. Cancer, 2006;119(10):2313-21.
Species: Human
Sample Type: Cell Culture Supernates

Modulation of GM-CSF release by enantiomers of beta-agonists in human airway smooth muscle.
Authors: Ameredes BT, Calhoun WJ
J. Allergy Clin. Immunol., 2005;116(1):65-72.
Species: Human
Sample Type: Whole Cells

Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions.
Authors: Bartemes KR, Cooper KM, Drain KL, Kita H
J. Allergy Clin. Immunol., 2005;116(4):827-35.
Species: Human
Sample Type: Cell Culture Supernates

Presence of dendritic cells, MCP-1, and activated microglia/macrophages in amyotrophic lateral sclerosis spinal cord tissue.
Authors: Henkel JS, Engelhardt JI, Siklos L, Simpson EP, Kim SH, Pan T, Goodman JC, Siddique T, Beers DR, Appel SH
Ann. Neurol., 2004;55(2):221-35.
Species: Human
Sample Type: CSF

Effect of ozone exposure on airway responses to inhaled allergen in asthmatic subjects.
Authors: Chen LL, Tager IB, Peden DB, Christian DL, Ferrando RE, Welch BS, Balmes JR
Chest, 2004;125(6):2328-35.
Species: Human
Sample Type: Airway Lavage

Specific recognition and killing of glioblastoma multiforme by interleukin 13-zetakine redirected cytolytic T cells.
Authors: Kahlon KS, Brown C, Cooper LJ, Raubitschek A, Forman SJ, Jensen MC
Cancer Res., 2004;64(24):9160-6.
Species: Human
Sample Type: Cell Culture Supernates

Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
Authors: Hellman C, Hallden G, Hylander B, Lundahl J
Clin. Exp. Immunol., 2003;131(1):75-81.
Species: Human
Sample Type: Peritoneal Fluid

Spontaneous circulation of myeloid-lymphoid-initiating cells and SCID-repopulating cells in sickle cell crisis.
Authors: Lamming CE, Augustin L, Blackstad M, Lund TC, Hebbel RP, Verfaillie CM
J. Clin. Invest., 2003;111(6):811-9.
Species: Human
Sample Type: Serum

Thymus and activation-regulated chemokine (TARC/CCL17) produced by mouse epidermal Langerhans cells is upregulated by TNF-alpha and IL-4 and downregulated by IFN-gamma.
Authors: Xiao T, Fujita H, Saeki H, Mitsui H, Sugaya M, Tada Y, Kakinuma T, Torii H, Nakamura K, Asahina A, Tamaki K
Cytokine, 2003;23(4):126-32.
Species: Human
Sample Type: Serum

Autocrine type I IFN and contact with endothelium promote the presentation of influenza A virus by monocyte-derived APC.
Authors: Qu C, Moran TM, Randolph GJ
J. Immunol., 2003;170(2):1010-8.
Species: Human
Sample Type: Cell Culture Supernates

Retroviral interleukin 1alpha gene transfer in bone marrow stromal cells in a primate model: induction of myelopoiesis stimulation.
Authors: de Revel T, Becard N, Sorg T, Rousseau S, Spano JP, Thiebot H, Methali M, Gras G, Le Grand R, Dormont D
Br. J. Haematol., 2002;118(3):875-84.
Species: Primate- Macaca fascicularis (Cynomolgus/Crab-eating Monkey)
Sample Type: Cell Culture Supernates

Local immunostimulation induced by intravesical administration of autologous interferon-gamma-activated macrophages in patients with superficial bladder cancer.
Authors: Pages F, Lebel-Binay S, Vieillefond A, Deneux L, Cambillau M, Soubrane O, Debre B, Tardy D, Lemonne JL, Abastado JP, Fridman WH, Thiounn N
Clin. Exp. Immunol., 2002;127(2):303-9.
Species: Human
Sample Type: Urine

Distinct hematopoietic support by two human stromal cell lines.
Authors: Loeuillet C, Bernard G, Remy-Martin J, Saas P, Herve P, Douay L, Chalmers D
Exp. Hematol., 2001;29(6):736-45.
Species: Human
Sample Type: Cell Culture Supernates

c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin.
Authors: Szabowski A, Maas-Szabowski N, Andrecht S, Kolbus A, Schorpp-Kistner M, Fusenig NE, Angel P
Cell, 2000;103(5):745-55.
Species: Human
Sample Type: Cell Culture Supernates

Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
J. Exp. Med., 2000;192(2):219-26.
Species: Human
Sample Type: Cell Culture Supernates

Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
Authors: Sato E, Koyama S
Eur. Respir. J., 2000;16(5):951-8.
Species: Human
Sample Type: Cell Culture Supernates

Serum levels of interleukins, growth factors and angiogenin in patients with endometrial cancer.
Authors: Chopra V, Dinh TV, Hannigan EV
J. Cancer Res. Clin. Oncol., 1997;123(3):167-72.
Species: Human
Sample Type: Serum

High EMT Signature Score of Invasive Non-Small Cell Lung Cancer (NSCLC) Cells Correlates with NFkappaB Driven Colony-Stimulating Factor 2 (CSF2/GM-CSF) Secretion by Neighboring Stromal Fibroblasts.
Authors: Rudisch A, Dewhurst M, Horga L, Kramer N, Harrer N, Dong M, van der Kuip H, Wernitznig A, Bernthaler A, Dolznig H, Sommergruber W
PLoS ONE, 0;10(4):e0124283.
Species: Human
Sample Type: Cell Culture Supernates

生物活性< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
背景GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.
运输条件Blue Ice
存放说明4℃
参考文献
  • Common beta Chain Receptor Family
  • Cytokines and Receptors in Allergy and Asthma
  • Cytokines and Receptors in Angiogenesis
  • Dendritic Cell Development
  • Gametes and Fertilization
  • gamma delta T Cells
  • Inflammatory Mediators
  • Isolation, Culture, and Identification of Natural Killer Cells
  • Macrophage Activation Markers
  • Myeloid-derived Suppressor Cells (MDSC)
  • Natural Killer T (NKT) Cells
  • T Cell Cytokine Signaling