| 货号 | K4217-100 |
| 描述 | Phospho- SHP2 (Y546) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated SHP2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-SHP2. An anti-pan SHP2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SHP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-SHP2 (Y546) antibody is used to detect phosphorylated SHP2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SHP2 (Y546) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 供应商 | Biovision |
| 样本类型以及上样量 | • Cell Lysates |
| 试剂盒组分 | • Microplate (Item A) coated with anti-pan SMAD1 antibody • Wash Buffer Concentrate (20x) (Item B) • Assay Diluent (5X) (Item E2) • Detection Antibody SHP2 (Y542) (Item C-1) • HRP-conjugated Anti-rabbit IgG (Item D-1) • TMB One-Step Substrate Reagent (Item H) • Stop Solution (Item I) • Cell Lysate Buffer (2X) (Item J) • Positive Control 3T3S001-1 (Item K): lyophilized powder from NIH3T3 cell lysate |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |