Periostin/OSF-2 (human) ELISA Kit
货号：
K4760-100 基本售价：
10520.0 元 规格：
100assays
产品信息
概述| 货号 | K4760-100 |
| 描述 | BioVision’s Periostin (human) ELISA kit is to be used for the in vitro quantitative determination of human periostin in cell culture supernatants, serum and plasma. A monoclonal antibody specific for periostin has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’- tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of periostin in the samples. Detection limit: 15 pg/ml. Note: The Limit of detection was measured by adding three standard deviations to the mean value of 50 zero standard. Assay Range: 78 pg/ml – 5000 pg/ml. Linearity: Different samples containing human periostin were diluted several fold (1/50 to 1/100) and the measured recoveries ranged from 95% to 105%. Human periostin levels range in plasma or serum from 10 to >100 ng /ml. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Species reactivity - human, mouse• Applications - Quantitative ELISA |
| 样本类型以及上样量 | • Serum & plasma • Cell culture supernatants |
| 产品特色 | • Easy, convenient and time-saving method to assay Periostin. • The minimum detectable concentration of periostin is 15 pg/ml. • This ELISA is specific for the measurement of natural and recombinant human periostin. It has been tested on human periostin isoforms 1 and 2 (should detect human isoforms 3 and 4). It does cross-react with mouse periostin. |
| 试剂盒组分 | • Human periostin Standard (Lyophilized) (1 μg) (STD)• Detection Antibody (20 μl) (DET)• HRP Labeled Streptavidin (lyophilized) (2 μg) (STREP-HRP)• 10X Wash Buffer (2 X 30 ml)• 10X ELISA Buffer (2 X 30 ml) • TMB Substrate Solution (12 ml) (TMB)• Stop Solution (12 ml) (STOP)• Plate coated with periostin Antibody (6 X 16-well strips)• Plate Covers (plastic film)• Silica Gel Minibags |
| 运输条件 | Blue Ice |
| 存放说明 | +4℃ |
