RBP4 (human) ELISA Kit
货号：
K4912-100 基本售价：
10220.0 元 规格：
100assays
产品信息
概述| 货号 | K4912-100 |
| 描述 | Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Adipocyte-specific deletion of Gluts leads to notable elevation of RBP4 causing systemic insulin resistance, and that reduction of RBP4 improves insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocyte-derived hormone. The RBP4 (human) ELISA Kit is to be used for the in vitro quantitative determination of human RBP4 in biological fluids. This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG, peroxidase and TMB (3,3’,5,5’-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of RBP4 in the samples. This ELISA is specific for the measurement of natural and recombinant human RBP4. It does not cross-react with mouse RBP4, rat RBP4, human adiponectin, human resistin, human vaspin, human clusterin, human leptin, human IL-33, human GPX3, human progranulin, human FABP4, human ANGPTL3, human ANGPTL4, human ANG1, human ANG2, human Nampt, mouse Nampt. The assay range is 0.39 – 25 ng RBP4/ml and a detection limit of 380 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards). |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Sample type- Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications- This assay detects human RBP4 in the range of 0.39 |
| 产品特色 | • Simple procedure • Fast and convenient • High-throughput adaptable |
| 试剂盒组分 | • Pre-coated Microtiter Plate • Wash Buffer (10X) • ELISA Buffer (10X) • Detection Antibody • Detector 100X (HRP conjugated anti-rabbit IgG) • Human RBP4 Standard (lyophilized, 50 ng) • TMB Substrate Solution • Stop Solution• Plate Sealers• Silica Gel Minibags |
| 运输条件 | Blue Ice |
| 存放说明 | +4℃ |
