| 货号 | K4215-100 |
| 描述 | Phospho-INSR ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phospho-INSR in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-INSR. An anti-INSR antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated INSR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-INSR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 供应商 | Biovision |
| 样本类型以及上样量 | • Cell Lysates |
| 试剂盒组分 | • Microplate (Item A) coated with anti-INSR antibody • Wash Buffer Concentrate (20x) (Item B) • Assay Diluent (5X) (Item E2) • Biotinylated anti-phosphotyrosine (Item C) • HRP-Streptavidin Concentrate (600X) (Item G) • TMB One-Step Substrate Reagent (Item H) • Stop Solution (Item I) • Cell Lysate Buffer (2X) (Item J) • Positive Control JktPV001-2 (Item K): lyophilized powder from cell lysate |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |