| 货号 | K4805-100 |
| 描述 | BioVision’s Human Carcinoembryonic Antigen (CEA) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CEA. This assay employs an antibody specific for human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CEA is typically less than 200 pg/ml. Detection Range: 0.2 ng/ml – 250 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. |
| 同种亚型 | Goat IgG |
| 来源宿主 | Goat |
| 供应商 | Biovision |
| 样本类型以及上样量 | • Serum & plasma • Cell culture supernatants • Urine |
| 试剂盒组分 | • CEA Microplate (Item A) coated with anti-human CEA, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Standards (Item C) (recombinant human CEA) • Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent. 0.09% sodium azide as preservative • Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. 5x concentrated • Detection Antibody CEA (Item F), biotinylated anti-human CEA (each vial enough for half plate) • HRP-Streptavidin Concentrate (Item G), 1000x concentrated • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |