IL-1β (rat) ELISA Kit
货号：
K4796-100 基本售价：
9275.0 元 规格：
100assays
产品信息
概述| 货号 | K4796-100 |
| 描述 | BioVision’s rat IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta. This assay employs an antibody specific for IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Sensitivity: 80 pg/ml. Detection Limit: 80 pg/ml – 50000 pg/ml. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Species reactivity - rat• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results |
| 样本类型以及上样量 | • Serum & plasma• Cell culture supernatants |
| 产品特色 | • Easy, convenient and time-saving method to assay IL-1 beta.• The minimum detectable concentration of IL-1 beta is less than 80 pg/ml. • This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α. |
| 试剂盒组分 | • IL-1 beta Microplate (Item A) coated with anti-rat IL-1 beta, 96 wells• Wash Buffer Concentrate (20x) (Item B)• Standards (Item C) (recombinant IL-1 beta)• Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative)• Assay Diluent B (Item E) for Standard/Sample (cell culture medium) diluent (5x concentrated)• Detection Antibody IL-1 beta (Item F), biotinylated anti- IL-1 beta (each vial enough for half plate) • HRP-Streptavidin Concentrate (Item G), 200x concentrated• TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
