| 货号 | K4773-100 |
| 描述 | BioVision’s Human IFN-γ ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IFN-γ. This assay employs an antibody specific for human IFN-γ coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-γ present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IFN-γ antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of IFN-γ is typically less than 15 pg/ml. Detection Range: 15 pg/ml - 15000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Species reactivity - human• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results |
| 样本类型以及上样量 | • Serum & plasma (human IFN-γ concentration is pretty low in normal serum/plasma, it may not be detected in this assay)• Cell culture supernatants• Urine |
| 产品特色 | • Easy, convenient and time-saving method to assay IFN-gamma.• The minimum detectable concentration of IFN-gamma is less than 15 pg/ml. • This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. |
| 试剂盒组分 | • IFN-γ Microplate (Item A) coated with anti-human IFN-γ, 96 wells• Wash Buffer Concentrate (20x) (Item B)• Standards (Item C) (recombinant human IFN-γ)• Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) • Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated)• Detection Antibody IFN-γ (Item F), biotinylated anti-human IFN-γ (each vial enough for half plate)• HRP-Streptavidin Concentrate (Item G), 400x concentrated• TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |