| 产品特色 | • Easy, convenient and time-saving method to assay for mouse TPO. • The minimum detectable dose of TPO is typically less than 30 pg/ml. • This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13,IL-17, IFN-γ, KC, Leptin, MCP-1,MCP-5, MIP-1α, MIP-2, MIP-3β, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-α, VEGF). |
| 试剂盒组分 | • TPO Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mouse TPO. • Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. • Standards (Item C): 2 vials, recombinant mouse TPO. • Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium) diluent. • Detection Antibody TPO (Item F): 2 vial of biotinylated anti-mouse TPO (each vial is enough to assay half microplate). • HRP-Streptavidin Concentrate (Item G): 200 µl of 300x concentrated HRP-conjugated streptavidin. • TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. • Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. |
