| 货号 | K4750-100 |
| 描述 | BioVision’s Mouse TIMP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TIMP-1. This assay employs an antibody specific for mouse TIMP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TIMP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of TIMP-1 is typically less than 3 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Species reactivity- Mouse • Applications- Quantitative protein detection, establishing normal range, validation of antibody array results. |
| 样本类型以及上样量 | • Serum & plasma • Cell culture supernatants |
| 产品特色 | • Easy, convenient and time-saving method to assay for mouse TIMP-1. • The minimum detectable dose of TIMP-1 is typically less than 3 pg/ml. • This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF). |
| 试剂盒组分 | • TIMP-1 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mouse TIMP-1. • Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. • Standards (Item C): 2 vials, recombinant mouse TIMP-1. • Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium) diluent. • Detection Antibody TIMP-1 (Item F): 2 vial of biotinylated anti-mouse TIMP-1 (each vial is enough to assay half microplate). • HRP-Streptavidin Concentrate (Item G): 200 µl of 120x concentrated HRP-conjugated streptavidin. • TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. • Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |