MMP-8 (rat) ELISA Kit
货号：
K4743-100 基本售价：
9275.0 元 规格：
100assays
产品信息
概述| 货号 | K4743-100 |
| 描述 | BioVision’s Rat MMP-8 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MMP-8. This assay employs an antibody specific for MMP-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat MMP-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of MMP-8 is typically less than 50 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Species reactivity- Rat |
| 样本类型以及上样量 | • Serum & plasma (using heparin as an anticoagulant, EDTA & Citrate are not recommended) • Cell culture supernatants • Urine |
| 产品特色 | • Easy, convenient and time-saving method to assay for rat MMP-8. • The minimum detectable dose of MMP-8 is typically less than 50 pg/ml. • This ELISA kit is specific for the measurement of rat MMP-8. The kit shows no cross-reactivity with rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α. |
| 试剂盒组分 | • MMP-8 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-rat MMP-8. • Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. • Standards (Item C): 2 vials, recombinant rat MMP-8. • Assay Diluent A (Item D): 30 ml of diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. • Detection Antibody MMP-8 (Item F): 2 vial of biotinylated anti-MMP-8 (each vial is enough to assay half microplate). • HRP-Streptavidin Concentrate (Item G): 200 µl of 160x concentrated HRP-conjugated streptavidin. • TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. • Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
