| 货号 | K3073-100 |
| 描述 | BioVision’s mouse CD40 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD40. This assay employs an antibody specific for mouse CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fractalkine, GCSF, GM-CFS, IFN- γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13,IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF. The minimum detectable dose of CD40 is typically less than 3 pg/ml. Detection Range: 3 pg/ml – 1500 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Species reactivity - mouse• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results |
| 样本类型以及上样量 | • Serum & plasma • Cell culture supernatants |
| 产品特色 | • Easy, convenient and time-saving method to assay for mouse CD40. • The minimum detectable dose of CD40 is typically less than 3 pg/ml. • This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fractalkine, GCSF, GM-CFS, IFN- γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13,IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, M, CP-5, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| 试剂盒组分 | • CD40 Microplate (Item A) coated with anti-mouse CD40, 96 wells• Wash Buffer Concentrate (20x) (Item B)• Standards (Item C) (recombinant mouse CD40)• Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent. 0.09% sodium azide as preservative• Assay Diluent B (Item E) for Standard/Sample (cell culture medium) diluent. 5x concentrated• Detection Antibody CD40 (Item F), biotinylated anti-mouse CD40 (each vial enough for half plate) • HRP-Streptavidin Concentrate (Item G), 500x concentrated• TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |