| 货号 | K3072-100 |
| 描述 | BioVision’s Human CD40 (TNFRSF5) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD40. This assay employs an antibody specific for human CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin (OB), MCP-1, MDC, MIP-1α, MIP-1 β, MIP-1δ, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CD40 is typically less than 50 pg/ml. Detection Range: 50 pg/ml – 40000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Species reactivity - human• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results |
| 样本类型以及上样量 | • Serum & plasma • Cell culture supernatants• Urine |
| 产品特色 | • Easy, convenient and time-saving method to assay for human CD40. • The minimum detectable dose of CD40 is typically less than 50 pg/ml. • This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin (OB), MCP-1, MDC, MIP-1α, MIP-1 β, MIP-1δ, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. |
| 试剂盒组分 | • CD40 Microplate (Item A) coated with anti-human CD40, 96 wells• Wash Buffer Concentrate (20x) (Item B)• Standards (Item C) (recombinant human CD40)• Assay Diluent D (Item K) for Standard/Sample (serum/plasma/cell culture medium/urine) diluent. 5x concentrated• Assay Diluent B (Item E) for detection antibody & HRP-Streptavidin diluent. 5x concentrated• Detection Antibody CD40 (Item F), biotinylated anti-human CD40 (each vial enough for half plate) • HRP-Streptavidin Concentrate (Item G), 500x concentrated• TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |