| 货号 | K7568-100 |
| 描述 | BioVision’s Mouse Lipocalin-2 (LCN2)/NGAL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Lipocalin-2. This assay employs an antibody specific for mouse Lipocalin-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Lipocalin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Lipocalin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lipocalin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33, IL-7 R alpha, Kremen-1, Limitin, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40, Ligand, P-Cadherin, Periostin, PlGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon. Sensitivity: 0.02 ng/ml. Detection Range: 0.02 ng/ml – 100 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 供应商 | Biovision |
| 样本类型以及上样量 | • Serum & plasma • Cell culture supernatants |
| 试剂盒组分 | • Plate coated with anti-mouse Lipocalin-2 • Wash Buffer (20x) (Item B) • Mouse Lipocalin-2 Standard (Item C) • Assay Diluent (Item E) (5x) • Detection Ab Lipocalin-2 (Item F) • HRP-Streptavidin (Item G) (500x) • TMB (Item H) • Stop Solution (Item I) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |