Progranulin (human) ELISA Kit
货号：
K4738-100 基本售价：
9033.0 元 规格：
100assays
产品信息
概述| 货号 | K4738-100 |
| 描述 | BioVision’s Progranulin (human) ELISA kit is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of progranulin in the samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Species reactivity- Human |
| 样本类型以及上样量 | • Serum • Plasma • Urine • Cell culture supernatants • Cerebrospinal Fluid (CSF) |
| 产品特色 | • The lowest level of progranulin that can be detected by this assay is 32 pg/ml. • The Assay range is 0.063 ng/ml – 4 ng/ml • This ELISA is specific for the measurement of natural and recombinant human progranulin. It does not cross-react with human RBP4, human adiponectin, human Nampt, human leptin, human RELM-β, human ANGPTL6, human FABP4, human TNF-α, human IL-33, human GPX3, human resistin, human clusterin, human vaspin, human PAl1, human ANGPTL3, mouse Nampt, rat Nampt. |
| 试剂盒组分 | • 1 plate coated with human progranulin Antibody (6 x 16-well strips) • 2 bottle Wash Buffer 10X (30 ml) • 2 bottle ELISA 10X (30 ml) • 1 vial Detection Antibody (30 μl) • 1 vial HRP Labeled Streptavidin (lyophilized) (2 μl) • 1 vial human progranulin Standard (lyophilized) (8 ng) • 1 bottle TMB Substrate Solution (12 ml) • 1 bottle Stop Solution (12 ml) • 2 Silica Gel Minibags • 2 plate sealers (plastic film) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
