Adiponectin (rat) Elisa Assay Kit
货号:
K4903-100 基本售价:
9033.0 元 规格:
1x96assays
产品信息
概述货号 | K4903-100 |
描述 | Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin/gACRP30 in regulation of glucose and/or lipid metabolism. This Rat Adiponectin/gACRP30 ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin/gACRP30 in rat serum, plasma or various tissue or cell culture supernatants. In the assay, polyclonal antibody specific for rat adiponectin/gACRP30 has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and any adiponectin present is bound by immobilized antibody. The bound adiponectin/gACRP30 is then captured by anti-rat adiponectin/gACRP30 monoclonal antibody. With adding HRP conjugated mouse IgG and HRP substrate, the colors developed in proportion to the bound adiponectin/gACRP30, can be easily measured by Elisa plate reader. |
同种亚型 | Goat IgG |
来源宿主 | Goat |
性能供应商 | Biovision |
检测类型 | Absorbance (450 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications- This rat Adiponectin/gACRP30 ELISA Kit is an e |
产品特色 | • Simple procedure • Fast and convenient |
试剂盒组分 | • Antibody coated 96-well plate (12x8 well strips) • 10X Wash concentrate, 50 ml • 5X Diluent, 50 ml • Secondary antibody, 12 ml • 100X Detector (HRP conjugated mouse IgG), 150 µl • Standard (recombinant rat adiponectin from HEK 293cells), 1 vial, lyophilized (48 ng) • QC sample= a positive control of rat serum adiponectin, 1 vial, lyophilized (12.67 μg) • Substrate I, 6 ml • Substrate II, 6 ml • Stop solution, 12 ml |
运输条件 | Blue Ice |
存放说明 | +4℃ |