Progesterone ELISA Kit
货号:
K7416-100 基本售价:
8851.5 元 规格:
100assays
产品信息
概述货号 | K7416-100 |
描述 | BioVision’s Progesterone ELISA kit is a competitive immunoassay for the quantitative determination of Progesterone in biological fluids. The kit uses a polyclonal antibody to Progesterone to bind, in a competitive manner, Progesterone in a sample or Progesterone which has an alkaline phosphatase molecule covalently attached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405 nm. The intensity of the bound yellow color is inversely proportional to the concentration of Progesterone in either standards or samples. The measured optical density for the samples is used to calculate the concentration of Progesterone in the sample. Sensitivity: 8.57 pg/ml (range: 15.62-500 pg/ml). The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Cross Reactivity: Progesterone:100%, 5-Pregnane-3,20-dione: 100%, 17-OH-Progesterone: 3.46%, 5 alpha-Pregnen-3ß-o1-20-one: 1.43%, Corticosterone: 0.77%, 4-Androstene-3,17-dione: 0.28%, Deoxycorticosterone: 0.056%, DHEA: 0.013%, 17ß-Estradiol: <0.001%, Estrone: 0.001%, Estriol: <0.001%, Testosterone: <0.001%, Hydrocortisone: <0.001%, 5 alpha-Pregnane-3,20-diol: <0.001%, Danazol: <0.001% |
同种亚型 | Rabbit IgG1 |
来源宿主 | Rabbit |
性能供应商 | Biovision |
检测类型 | Absorbance (450 nm)• Species reactivity - all• Applications - Quantitative detection of progesterone |
样本类型以及上样量 | • Serum, saliva, tissue culture media |
产品特色 | • Easy, convenient and time-saving method to measure Progesterone from all species• Sensitivity: 8.57 pg/ml (range: 15.62-500 pg/ml) |
试剂盒组分 | • Goat anti-Mouse IgG Plate• Progesterone Alkaline Phosphatase Conjugate• Progesterone Monoclonal Ab• Assay Buffer• Wash Buffer Concentrate• Progesterone Standard (100 ng/ml, in ethanol)• pNpp Substrate• Stop Solution• Steroid Displacement Reagent• Plate Sealer |
运输条件 | Blue Ice |
存放说明 | +4℃ |