CTLA4 /CD152 (Human) ELISA Kit
货号:
K4157-100 基本售价:
8851.5 元 规格:
100assays
产品信息
概述货号 | K4157-100 |
描述 | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HumanCTLA4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CTLA4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CTLA4 amount of sample captured in plate. |
同种亚型 | Rabbit IgG |
来源宿主 | Rabbit |
性能供应商 | Biovision |
检测类型 | Absorbance (450 nm)• Species reactivity - Human• Applications - Quantitative measurement of CTLA4 in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids. |
样本类型以及上样量 | • Human serum, plasma, tissue homogenates, culture supernatants and other biological fluids. |
产品特色 | • Easy, convenient and time-saving method to measure the level of CTLA4 inserum, plasma, tissue homogenates, culture supernatants and other biological fluids. |
试剂盒组分 | • Microplate: 96 wells, coated with anti-human CTLA4• Lyophilized Standard• Sample / Standard dilution buffer• Biotin- detection antibody (Concentrated)• Antibody dilution buffer• HRP-Streptavidin Conjugate (SABC)• SABC dilution buffer• TMB substrate• Stop Solution• Wash buffer (25X)• Plate sealers |
运输条件 | Blue Ice |
存放说明 | +4℃ |