Human Cytochrome c ELISA Kit (Colorimetric)
货号：
NBP1-91263 基本售价：
8308.3 元 规格：
96 Tests
产品信息
概述| 货号 | NBP1-91263 |
| 反应种属 | HuSpecies Glossary |
| 应用 | ELISA |
性能| 供应商 | Novus |
| 背景 | Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells. Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria. Measurement of cytochrome c release from the mitochondria is a tool to detect the first early steps for initiating apoptosis in cells. Moreover, elevated cytochrome c levels were observed in serum from patients with hematological malignancies. In the course of cancer chemotherapy, the serum-cytochrome c level grew rapidly and it decreased gradually as the patient was cleared from malignant cells. Thus, serum-cytochrome c monitoring might serve as a clinical marker indicating the onset of apoptosis and cell turn-over in vivo. |
| 存放说明 | Storage of components varies. See protocol for specific instructions. |
