VEGF (rat) ELISA Kit
货号：
K5365-100 基本售价：
8246.5 元 规格：
100assays
产品信息
概述| 货号 | K5365-100 |
| 描述 | Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. BioVision’s rat VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for rat VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat VEGF captured onto the plate. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 1 pg/ml. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Species reactivity - rat• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results |
| 样本类型以及上样量 | • Serum & plasma • Cell culture supernatants• Urine |
| 产品特色 | • Easy, convenient and time-saving method to detect VEGF in rat serum, plasma, urine or cell culture supernatants.• Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 1 pg/ml.• This ELISA kit shows no detectable cross-reactivity with other related proteins. |
| 试剂盒组分 | • Plate coated with anti-rat VEGF antibody, 96-wells• Rat VEGF standard (10 ng/vial)• Biotinylated anti-rat VEGF antibody• Avidin-Biotin-Peroxidase Complex (ABC)• Sample diluent buffer• Antibody diluent buffer• ABC diluent buffer• TMB color developing agent (Colorless)• TMB stop solution |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
