| 货号 | 14784C |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Total PD-L1 Sandwich ELISA Kit detects endogenous levels of PD-L1 protein as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9). |
| 存放说明 | 4C |
| 参考文献 | Dong, H. et al. (1999) Nat Med 5, 1365-9. Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34. Liang, S.C. et al. (2003) Eur J Immunol 33, 2706-16. Dong, H. et al. (2002) Nat Med 8, 793-800. Thompson, R.H. et al. (2006) Cancer Res 66, 3381-5. Pardoll, D.M. (2012) Nat Rev Cancer 12, 252-64. Taube, J.M. et al. (2012) Sci Transl Med 4, 127ra37. Lyford-Pike, S. et al. (2013) Cancer Res 73, 1733-41. Chen, B.J. et al. (2013) Clin Cancer Res 19, 3462-73. |
Figure 1. PD-L1 protein is detected from KARPAS-299 and SUP-M2 cell lines using the PathScan® Total PD-L1 Sandwich ELISA Kit #14784. The absorbance readings at 450 nm are shown in the top figure, while corresponding western blots using PD-L1 (E1L3N®) XP® Rabbit mAb #13684 are shown in the bottom figure. Cell Line Source: Dr. Abraham Karpas at the University of Cambridge. | |
Figure 2. The relationship between protein concentration of lysates from SUP-M2 cells and the absorbance at 450 nm as detected by the PathScan® Total PD-L1 Sandwich ELISA Kit #14784 is shown. Unstarved SUP-M2 cells (0.5-1.0 x 106) were harvested and then lysed. |
