| 货号 | DLAP00 |
| 描述 | The Quantikine Human LAP (TGF-beta 1) Immunoassay is a 4.5 hour solid phase ELISA designed to measure LAP (TGF-beta 1) in acid activated cell culture supernates, serum, plasma, and urine. It contains recombinant human LAP (TGF-beta 1) expressed by CHO cells and has been shown to quantitate the recombinant factor accurately. Results obtained using natural LAP (TGF-beta 1) showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit will provide accurate quantitation for both recombinant and natural human LAP (TGF-beta 1). |
| 别名 | LAP (TGFbeta 1); LAP (TGF-beta 1); | 全称 | Latency-associated Peptide |
| 反应种属 | Human |
| 目标/特异性 | Natural and recombinant human LAP (TGF-beta 1) and Latent TGF-beta 1). |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7040 (Human) |
| 生物活性 | < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested. |
| 背景 | Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus. TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic cysteine knot. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
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