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Human CXCL5/ENA-78 Quantikine ELISA Kit (1 KT)

货号: DX000 基本售价: 7217.7 元 规格: 1 Kit

产品信息

概述
货号DX000
描述The Quantikine Human ENA-78 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human ENA-78 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human ENA-78 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human ENA-78 accurately. Results obtained using natural human ENA-78 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human ENA-78.
别名CXCL5; ENA-78;
反应种属Human
目标/特异性Natural and recombinant human ENA-78
性能
供应商R&D Systems
检测类型Solid Phase Sandwich ELISA
Entrez Gene IDs
6374 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid.
Authors: Tzeng, Tsai-Ten, Tsay, Huey-Jen, Chang, Luping, Hsu, Chia-Lin, Lai, Tzu-Hsua, Huang, Fong-Lee, Shiao, Young-Ji
J Biomed Sci, 2013;20(0):90.
Species: Human
Sample Type: Cell Culture Supernates

Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
J. Thromb. Haemost., 2012;10(8):1616-23.
Species: Human
Sample Type: Serum

Inhibition of IL-1beta-mediated inflammatory responses by the IkappaB alpha super-repressor in human fibroblast-like synoviocytes.
Authors: Lee YR, Kweon SH, Kwon KB, Park JW, Yoon TR, Park BH
Biochem. Biophys. Res. Commun., 2009;378(1):90-4.
Species: Human
Sample Type: Cell Culture Supernates

IL-8 as antibody therapeutic target in inflammatory diseases: reduction of clinical activity in palmoplantar pustulosis.
Authors: Skov L, Beurskens FJ, Zachariae CO, Reitamo S, Teeling J, Satijn D, Knudsen KM, Boot EP, Hudson D, Baadsgaard O, Parren PW, van de Winkel JG
J. Immunol., 2008;181(1):669-79.
Species: Human
Sample Type: palmoplantar pustulosis wash fluid

Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
Authors: Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
Cancer Res., 2008;68(23):9996-10003.
Species: Human
Sample Type: Serum

Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion.
Authors: Flanagan K, Modrusan Z, Cornelius J, Chavali A, Kasman I, Komuves L, Mo L, Diehl L
J. Immunol., 2008;180(6):3874-81.
Species: Mouse
Sample Type: Cell Culture Supernates

Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes.
Authors: Lee YR, Lee JH, Noh EM, Kim EK, Song MY, Jung WS, Park SJ, Kim JS, Park JW, Kwon KB, Park BH
Life Sci., 2008;82(23):1203-9.
Species: Human
Sample Type: Cell Culture Supernates

CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
Authors: Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM
Biochem. Pharmacol., 2008;76(1):79-90.
Species: Human
Sample Type: Cell Culture Supernates

Th1/Th2 cytokines reciprocally regulate in vitro pulmonary angiogenesis via CXC chemokine synthesis.
Authors: Matsuda A, Fukuda S, Matsumoto K, Saito H
Am. J. Respir. Cell Mol. Biol., 2007;38(2):168-75.
Species: Human
Sample Type: Cell Culture Supernates

Expression and gene polymorphisms of the chemokine CXCL5 in colorectal cancer patients.
Authors: Dimberg J, Dienus O, Lofgren S, Hugander A, Wagsater D
Int. J. Oncol., 2007;31(1):97-102.
Species: Human
Sample Type: Plasma

L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
Atherosclerosis, 2007;192(1):56-66.
Species: Human
Sample Type: Cell Culture Supernates

Cooperative effects of rhinovirus and TNF-{alpha} on airway epithelial cell chemokine expression.
Authors: Newcomb DC, Sajjan US, Nagarkar DR, Goldsmith AM, Bentley JK, Hershenson MB
Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(4):L1021-8.
Species: Human
Sample Type: Cell Culture Supernates

Crucial role of the protein C pathway in governing microvascular inflammation in inflammatory bowel disease.
Authors: Scaldaferri F, Sans M, Vetrano S, Graziani C, De Cristofaro R, Gerlitz B, Repici A, Arena V, Malesci A, Panes J, Grinnell BW, Danese S
J. Clin. Invest., 2007;117(7):1951-60.
Species: Human
Sample Type: Cell Culture Supernates

Different angiogenic activity in pulmonary sarcoidosis and idiopathic pulmonary fibrosis.
Authors: Antoniou KM, Tzouvelekis A, Alexandrakis MG, Sfiridaki K, Tsiligianni I, Rachiotis G, Tzanakis N, Bouros D, Milic-Emili J, Siafakas NM
Chest, 2006;130(4):982-8.
Species: Human
Sample Type: BALF

IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
J. Immunol., 2005;175(9):6177-89.
Species: Human
Sample Type: Cell Culture Supernates

CXC-chemokine stimulation of neutrophils correlates with plasma levels of myeloperoxidase and lactoferrin and contributes to clinical outcome after pediatric cardiac surgery.
Authors: Gessler P, Pretre R, Hohl V, Rousson V, Fischer J, Dahinden C
Shock, 2004;22(6):513-20.
Species: Human
Sample Type: Plasma

Peritoneal fluid concentrations of epithelial neutrophil-activating peptide-78 correlate with the severity of endometriosis.
Authors: Suzumori N, Katano K, Suzumori K
Fertil. Steril., 2004;81(2):305-8.
Species: Human
Sample Type: Peritoneal Fluid

ELR+ CXC chemokines in human milk.
Authors: Maheshwari A, Christensen RD, Calhoun DA
Cytokine, 2003;24(3):91-102.
Species: Human
Sample Type: Serum

Evasion from NK cell immunity by MHC class I chain-related molecules expressing colon adenocarcinoma.
Authors: Doubrovina ES, Doubrovin MM, Vider E, Sisson RB, O'Reilly RJ, Dupont B, Vyas YM
J. Immunol., 2003;171(12):6891-9.
Species: Human
Sample Type: Serum

TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion.
Authors: Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M
Eur. J. Immunol., 2002;32(7):2037-45.
Species: Human
Sample Type: Cell Culture Supernates

生物活性< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
背景CXCL5/Epithelial Cell-derived Neutrophil-activating Peptide (ENA-78), is a member of the CXC subfamily of chemokines. Full-length CXCL5/ENA-78 is 114 amino acids (aa) in length with a predicted molecular weight of 12 kDa. Following the removal of the signal peptide, bioactive CXCL5/ENA-78 is 78 aa in length. CXCL5/ENA-78 can be N-terminally cleaved by Cathepsin G and Chymotrypsin to CXCL5/ENA-74 (74 aa) and CXCL5/ENA-70 (70 aa), which show increased potency relative to CXCL5/ENA-78. While murine LIX was thought to be an ortholog to human CXCL5/ENA-78, genome-wide analysis and a consensus in the field suggests that human CXCL5/ENA-78 does not have a true murine ortholog. CXCL5/ENA-78 is upregulated at sites of inflammation and is expressed by multiple hematopoietic cell types, fibroblasts, endothelial cells, vascular smooth muscle cells, and adipocytes. It acts as a chemoattractant for neutrophils, has angiogenic properties, and contributes to cancer progression.
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参考文献
  • Chemokines and Receptors
  • Chemokines and Receptors in Angiogenesis
  • CXC Chemokines and Receptors
  • Macrophage Activation Markers