| 货号 | DTM400 |
| 描述 | The Quantikine Human TIMP-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIMP-4 levels in cell culture supernates, serum, plasma, and human milk. It contains Sf 21-expressed recombinant human TIMP-4 and antibodies raised against the recombinant protein. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms. Natural human TIMP-4 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIMP-4. |
| 别名 | metalloproteinase inhibitor 4; TIMP metallopeptidase inhibitor 4; TIMP-4; tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4; | 全称 | Tissue Inhibitors of Metalloproteinases 4 |
| 反应种属 | Human |
| 目标/特异性 | Natural and recombinant human TIMP-4. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms. |
| 供应商 | R&D Systems |
| 检测类型 | Solid Phase Sandwich ELISA |
| Entrez Gene IDs | 7079 (Human); 110595 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Circulating biomarkers of tissue remodelling in pulmonary hypertension. | |
| 生物活性 | < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested. |
| 背景 | TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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