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Human TGF-alpha Quantikine ELISA Kit (1 KT)

货号: DTGA00 基本售价: 7217.7 元 规格: 1 Kit

产品信息

概述
货号DTGA00
描述The Quantikine Human TGF-alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TGF-alpha in cell culture supernates, serum, plasma, and human milk. It contains E. coli-expressed recombinant human TGF-alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TGF-alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TGF-alpha.
别名protransforming growth factor alpha; TFGA; TGFA; transforming growth factor, alpha; transforming growth factor-alpha;
全称
Transforming Growth Factor alpha
反应种属Human
目标/特异性Natural and recombinant human TGF-alpha
性能
供应商R&D Systems
检测类型Solid Phase Sandwich ELISA
Entrez Gene IDs
7039 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

DUOX-Mediated Signaling Is Not Required for LPS-Induced Neutrophilic Response in the Airways.
Authors: Chang S, Linderholm A, Harper R
PLoS ONE, 2015;10(7):e0131810.
Species: Mouse
Sample Type: BALF

Circulating hepatocyte growth factor is correlated with resistance to cetuximab in metastatic colorectal cancer.
Authors: Yonesaka, Kimio, Satoh, Taroh, Ueda, Shinya, Yoshida, Takeshi, Takeda, Masayuki, Shimizu, Toshio, Okamoto, Isamu, Nishio, Kazuto, Tamura, Takao, Nakagawa, Kazuhiko
Anticancer Res, 2015;35(3):1683-9.
Species: Human
Sample Type: Plasma

Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer.
Authors: Tanizaki J, Okamoto I, Okabe T, Sakai K, Tanaka K, Hayashi H, Kaneda H, Takezawa K, Kuwata K, Yamaguchi H, Hatashita E, Nishio K, Nakagawa K
Clin Cancer Res, 2012;18(22):6219-26.
Species: Human
Sample Type: cell culture supernate

ETS-1 oncogenic activity mediated by transforming growth factor alpha.
Authors: Holterman CE, Franovic A, Payette J
Cancer Res., 2010;70(2):730-40.
Species: Human
Sample Type: Cell Culture Supernates

Molecular response to cetuximab and efficacy of preoperative cetuximab-based chemoradiation in rectal cancer.
Authors: Debucquoy A, Haustermans K, Daemen A, Aydin S, Libbrecht L, Gevaert O, De Moor B, Tejpar S, McBride WH, Penninckx F, Scalliet P, Stroh C, Vlassak S, Sempoux C, Machiels JP
J. Clin. Oncol., 2009;27(17):2751-7.
Species: Human
Sample Type: Plasma

RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects.
Authors: Kreeger PK, Mandhana R, Alford SK, Haigis KM, Lauffenburger DA
Cancer Res., 2009;69(20):8191-9.
Species: Human
Sample Type: Cell Culture Supernates

Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer.
Authors: Han SW, Oh DY, Im SA, Park SR, Lee KW, Song HS, Lee NS, Lee KH, Choi IS, Lee MH, Kim MA, Kim WH, Bang YJ, Kim TY
Br. J. Cancer, 2009;100(2):298-304.
Species: Human
Sample Type: Serum

Cross-talk between estrogen receptor and epidermal growth factor receptor in head and neck squamous cell carcinoma.
Authors: Egloff AM, Rothstein ME, Seethala R, Siegfried JM, Grandis JR, Stabile LP
Clin. Cancer Res., 2009;15(21):6529-40.
Species: Human
Sample Type: Cell Culture Supernates

MMP-12 induces IL-8/CXCL8 secretion through EGFR and ERK1/2 activation in epithelial cells.
Authors: Le Quement C, Guenon I, Gillon JY, Lagente V, Boichot E
Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(6):L1076-84.
Species: Human
Sample Type: Cell Culture Supernates

Effective inhibition of the epidermal growth factor/epidermal growth factor receptor binding by anti-epidermal growth factor antibodies is related to better survival in advanced non-small-cell lung cancer patients treated with the epidermal growth factor cancer vaccine.
Authors: Garcia B, Neninger E, de la Torre A, Leonard I, Martinez R, Viada C, Gonzalez G, Mazorra Z, Lage A, Crombet T
Clin. Cancer Res., 2008;14(3):840-6.
Species: Human
Sample Type: Serum

Activation of the epidermal growth factor receptor (EGFR) by a novel metalloprotease pathway.
Authors: Bergin DA, Greene CM, Sterchi EE, Kenna C, Geraghty P, Belaaouaj A, Taggart CC, ONeill SJ, McElvaney NG
J. Biol. Chem., 2008;283(46):31736-44.
Species: Human
Sample Type: Cell Culture Supernates

Epidermal Growth Factor Receptor Blockade in Combination with Conventional Chemotherapy Inhibits Soft Tissue Sarcoma Cell Growth In vitro and In vivo.
Authors: Ren W, Korchin B, Zhu QS, Wei C, Dicker A, Heymach J, Lazar A, Pollock RE, Lev D
Clin. Cancer Res., 2008;14(9):2785-2795.
Species: Human
Sample Type: Cell Culture Supernates

Modification of the primary tumor microenvironment by transforming growth factor alpha-epidermal growth factor receptor signaling promotes metastasis in an orthotopic colon cancer model.
Authors: Sasaki T, Nakamura T, Rebhun RB, Cheng H, Hale KS, Tsan RZ, Fidler IJ, Langley RR
Am. J. Pathol., 2008;173(1):205-16.
Species: Human
Sample Type: Cell Culture Supernates

EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases.
Authors: Zhu J, Jia X, Xiao G, Kang Y, Partridge NC, Qin L
J. Biol. Chem., 2007;282(37):26656-64.
Species: Mouse
Sample Type: Cell Culture Supernates

Alteration of the serum levels of the epidermal growth factor receptor and its ligands in patients with non-small cell lung cancer and head and neck carcinoma.
Authors: Lemos-Gonzalez Y, Rodriguez-Berrocal FJ, Cordero OJ, Gomez C, Paez de la Cadena M
Br. J. Cancer, 2007;96(10):1569-78.
Species: Human
Sample Type: Serum

The response of human epithelial cells to TNF involves an inducible autocrine cascade.
Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
Cell, 2006;124(6):1225-39.
Species: Human
Sample Type: Cell Culture Supernates

IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
J. Immunol., 2005;175(9):6177-89.
Species: Human
Sample Type: Cell Culture Supernates

Mek1 alters epidermal growth and differentiation.
Authors: Scholl FA, Dumesic PA
Cancer Res., 2004;64(17):6035-40.
Species: Porcine
Sample Type: Cell Culture Supernates

生物活性< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
背景TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors with soluble forms released by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and plays a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. TGF-alpha binds and activates the EGF receptor. It induces fibroblast proliferation, epithelial development, keratinocyte migration, and angiogenesis.
运输条件Blue Ice
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参考文献
  • Cytokines and Receptors in Angiogenesis
  • EGF Family
  • EGF Ligands
  • HIF Transcription Factors
  • ICC / IHC Images: TGF-alpha
  • TGF-alpha: A New Job for an Old Friend