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Mouse VEGF R1/Flt-1 Quantikine ELISA Kit (1 KT)

货号: MVR100 基本售价: 6791.4 元 规格: 1 Kit

产品信息

概述
货号MVR100
描述The Quantikine Mouse soluble VEGF R1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R1.
别名EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGFR1; VEGFR-1;
全称
Vascular Endothelial Growth Factor Receptor 1
反应种属Mouse
目标/特异性Natural and recombinant mouse sVEGF R1
性能
供应商R&D Systems
检测类型Solid Phase Sandwich ELISA
Entrez Gene IDs
2321 (Human); 14254 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Reduced Uterine Perfusion Pressure (RUPP) Model of Preeclampsia in Mice
PLoS ONE, 2016;11(5):e0155426.
Species: Mouse
Sample Type: Plasma

Estrogen-related receptor gamma serves a role in blood pressure homeostasis during pregnancy.
Authors: Luo Y, Kumar P, Chen C, Latham J, Wang L, Tudela C, Alexander J, Shelton J, McKown L, Mendelson C
Mol Endocrinol, 2015;28(6):965-75.
Species: Mouse
Sample Type: Serum

Advances in the pathophysiology of pre-eclampsia and related podocyte injury.
Authors: Craici, Iasmina, Wagner, Steven J, Weissgerber, Tracey L, Grande, Joseph P, Garovic, Vesna D
Kidney Int, 2014;86(2):275-85.
Species: Rat
Sample Type:

Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
Authors: Suryawanshi A, Mulik S, Sharma S
J. Immunol., 2011;186(6):3653-65.
Species: Mouse
Sample Type: Tissue Homogenates

Role of reactive oxygen species during hypertension in response to chronic antiangiogenic factor (sFlt-1) excess in pregnant rats.
Authors: Tam Tam KB, Lamarca B, Arany M, Cockrell K, Fournier L, Murphy S, Martin JN, Granger JP
Am. J. Hypertens., 2011;24(1):110-3.
Species: Rat
Sample Type: Plasma

Sera from preeclampsia patients elicit symptoms of human disease in mice and provide a basis for an in vitro predictive assay.
Authors: Kalkunte S, Boij R, Norris W
Am. J. Pathol., 2010;177(5):2387-98.
Species: Mouse
Sample Type: Serum

Heterozygous deficiency of PHD2 restores tumor oxygenation and inhibits metastasis via endothelial normalization.
Authors: Mazzone M, Dettori D, Leite de Oliveira R, Loges S, Schmidt T, Jonckx B, Tian YM, Lanahan AA, Pollard P, Ruiz de Almodovar C, De Smet F, Vinckier S, Aragones J, Debackere K, Luttun A, Wyns S, Jordan B, Pisacane A, Gallez B, Lampugnani MG, Dejana E, Simons M, Ratcliffe P, Maxwell P, Carmeliet P
Cell, 2009;136(5):839-51.
Species: Mouse
Sample Type: Cell Culture Supernates

Novel role for vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.
Authors: Poesen K, Lambrechts D, Van Damme P, Dhondt J, Bender F, Frank N, Bogaert E, Claes B, Heylen L, Verheyen A, Raes K, Tjwa M, Eriksson U, Shibuya M, Nuydens R, Van Den Bosch L, Meert T, DHooge R, Sendtner M, Robberecht W, Carmeliet P
J. Neurosci., 2008;28(42):10451-9.
Species: Rat
Sample Type: Cell Lysates

An Adam15 amplification loop promotes vascular endothelial growth factor-induced ocular neovascularization.
Authors: Xie B, Shen J, Dong A, Swaim M, Hackett SF, Wyder L, Worpenberg S, Barbieri S, Campochiaro PA
FASEB J., 2008;22(8):2775-83.
Species: Mouse
Sample Type: Tissue Homogenates

Angiotensin receptor agonistic autoantibodies induce pre-eclampsia in pregnant mice.
Authors: Zhou CC, Zhang Y, Irani RA, Zhang H, Mi T, Popek EJ, Hicks MJ, Ramin SM, Kellems RE, Xia Y
Nat. Med., 2008;14(8):855-62.
Species: Mouse
Sample Type: Plasma

(Pro)renin receptor promotes choroidal neovascularization by activating its signal transduction and tissue renin-angiotensin system.
Authors: Satofuka S, Ichihara A, Nagai N, Noda K, Ozawa Y, Fukamizu A, Tsubota K, Itoh H, Oike Y, Ishida S
Am. J. Pathol., 2008;173(6):1911-8.
Species: Mouse
Sample Type: Tissue Homogenates

Inhibition of choroidal neovascularization with an anti-inflammatory carotenoid astaxanthin.
Authors: Izumi-Nagai K, Nagai N, Ohgami K, Satofuka S, Ozawa Y, Tsubota K, Ohno S, Oike Y, Ishida S
Invest. Ophthalmol. Vis. Sci., 2008;49(4):1679-85.
Species: Mouse
Sample Type: Tissue Homogenates

Soluble vascular endothelial growth factor receptor-1 protects mice in sepsis.
Authors: Tsao PN, Chan FT, Wei SC, Hsieh WS, Chou HC, Su YN, Chen CY, Hsu WM, Hsieh FJ, Hsu SM
Crit. Care Med., 2007;35(8):1955-60.
Species: Mouse
Sample Type: Plasma

Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy.
Authors: Shimojo N, Jesmin S, Zaedi S, Otsuki T, Maeda S, Yamaguchi N, Aonuma K, Hattori Y, Miyauchi T
Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H474-81.
Species: Rat
Sample Type: Cell Culture Supernates

Exercise training improves aging-induced downregulation of VEGF angiogenic signaling cascade in hearts.
Authors: Iemitsu M, Maeda S, Jesmin S, Otsuki T, Miyauchi T
Am. J. Physiol. Heart Circ. Physiol., 2006;291(3):H1290-8.
Species: Rat
Sample Type: Tissue Homogenates

Blockade of VEGF accelerates proteinuria, via decrease in nephrin expression in rat crescentic glomerulonephritis.
Authors: Hara A, Wada T, Furuichi K, Sakai N, Kawachi H, Shimizu F, Shibuya M, Matsushima K, Yokoyama H, Egashira K, Kaneko S
Kidney Int., 2006;69(11):1986-95.
Species: Mouse VEGF R1 transgenic rats
Sample Type: Urine

Selective suppression of pathologic, but not physiologic, retinal neovascularization by blocking the angiotensin II type 1 receptor.
Authors: Nagai N, Noda K, Urano T, Kubota Y, Shinoda H, Koto T, Shinoda K, Inoue M, Shiomi T, Ikeda E, Tsubota K, Suda T, Oike Y, Ishida S
Invest. Ophthalmol. Vis. Sci., 2005;46(3):1078-84.
Species: Mouse
Sample Type: Tissue Homogenates

Transduction of soluble Flt-1 gene to peritoneal mesothelial cells can effectively suppress peritoneal metastasis of gastric cancer.
Authors: Sako A, Kitayama J, Koyama H, Ueno H, Uchida H, Hamada H, Nagawa H
Cancer Res., 2004;64(10):3624-8.
Species: Mouse
Sample Type: Peritoneal Fluid

Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia.
Authors: Maynard SE, Min JY, Merchan J, Lim KH, Mondal S, Stillman IE, Epstein FH, Karumanchi SA
J. Clin. Invest., 2003;111(5):649-58.
Species: Rat
Sample Type: Plasma

生物活性< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
背景

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

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参考文献
  • Airway Inflammation and Remodeling
  • Cancer Biomarkers
  • Endothelial Cell Differentiation Markers
  • Endothelial Cell Markers
  • Endothelial Progenitor and Endothelial Cell Markers
  • HIF Transcription Factors
  • ICC / IHC Images: VEGF R1/Flt-1
  • Mini-review: Vascular Endothelial Growth Factor (VEGF)
  • Myeloid-derived Suppressor Cells (MDSC)
  • Platelets
  • Preeclampsia
  • Proliferation Bioassay HUVECs
  • Receptor Tyrosine Kinases (RTKs)
  • Receptor Tyrosine Kinases (RTKs) in the Akt Pathway
  • Receptors in the Jak/STAT Pathway
  • Technical Notes: The Quantikine ELISA in Establishing a Role for Endoglin in Preeclampsia
  • VEGF Family