| 货号 | MSHH00 |
| 描述 | The Quantikine Mouse Sonic Hedgehog N-Terminus Immunoassay is a 4.5 hour solid phase ELISA designed to measure Shh-N in cell culture supernates, serum, and plasma. It contains recombinant mouse Shh-N and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Shh-N showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Shh-N. |
| 别名 | HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; sonic hedgehog; TPT; TPTPS; |
| 反应种属 | Mouse |
| 目标/特异性 | Natural and recombinant mouse Shh-N. This kit also recognizes low levels of Shh-N in rat serum. |
| 供应商 | R&D Systems |
| 检测类型 | Solid Phase Sandwich ELISA |
| Entrez Gene IDs | 6469 (Human); 20423 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Spatially heterogeneous choroid plexus transcriptomes encode positional identity and contribute to regional CSF production. | |
| 生物活性 | < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested. |
| 背景 | Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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