| 货号 | MRBP40 |
| 描述 | The Quantikine Mouse RBP4 immunoassay is a 4.5 hour solid-phase ELISA designed to measure RBP4 in cell culture supernates, cell lysates, mouse serum, plasma, and urine. It contains NS0-expressed recombinant mouse RBP4 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant mouse RBP4. Results obtained using natural mouse RBP4 showed dose response curves that were parallel to the standard curve obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse RBP4. |
| 别名 | interstitial; Plasma retinol-binding protein; retinol binding protein 4, plasma; retinol-binding protein 4, plasma; |
| 反应种属 | Mouse |
| 目标/特异性 | Natural and recombinant mouse RBP4 |
| 供应商 | R&D Systems |
| 检测类型 | Solid Phase Sandwich ELISA |
| Entrez Gene IDs | 5950 (Human); 19662 (Mouse); 25703 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Serum retinol-binding protein-induced endothelial inflammation is mediated through the activation of toll-like receptor 4 | |
| 生物活性 | < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested. |
| 背景 | Retinol-binding protein 4 (RBP4) is a Lipocalin superfamily molecule that transports vitamin A (retinol) and retinaldehyde in the serum. Retinaldehyde is the critical chromophore in the rhodopsin photoreceptor, while both it and retinoic acid regulate a multitude of cellular differentiation and proliferation effects through the intracellular receptors RAR and RXR. RBP4 is secreted primarily by hepatocytes and adipocytes into the blood where the RBP4-retinol complex interacts with transthyretin (TTR). Formation of the complex with TTR increases the serum half-life of RBP4 by preventing RBP4 filtration through the kidney. The C-terminally processed forms of RBP4, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. RBP4 promotes hyperglycemia, and its upregulation in visceral and liver adipocytes leads to elevated serum levels. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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