货号 | MBB00 |
描述 | The Quantikine Mouse/Rat PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse/rat PDGF-BB in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat PDGF-BB and antibodies raised against recombinant human PDGF-BB. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse/rat PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat PDGF-BB. |
别名 | PDGFBB; PDGF-BB; | 全称 | Platelet-derived Growth Factor BB |
反应种属 | Mouse/Rat |
目标/特异性 | Natural and recombinant mouse and rat PDGF-BB. This assay cross-reacts 10.2% with recombinant Rat PDGF-AB, 5.3% with recombinant Human PDGF-AB, 64.7% with recombinant Human PDGF-BB, 38.1% with natural Human PDGF and 65.2% with natural Porcine PDGF. |
供应商 | R&D Systems |
检测类型 | Solid Phase Sandwich ELISA |
Entrez Gene IDs | 5155 (Human); 24628 (Rat) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts | |
生物活性 | Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested. |
背景 | The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta). |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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