| 货号 | RHMTA0 |
| 描述 | The Quantikine Rhesus Macaque TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure rhesus macaque TNF-alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rhesus macaque TNF-alpha and antibodies raised against recombinant rhesus macaque TNF-alpha. This immunoassay has been shown to quantitate the recombinant rhesus macaque TNF-alpha accurately. Results obtained using natural rhesus macaque TNF-alpha showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rhesus macaque TNF-alpha. |
| 别名 | APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha; | 全称 | Tumor Necrosis Factor alpha |
| 目标/特异性 | Natural and recombinant rhesus macaque TNF-alpha. This assay also recognizes recombinant Human TNF-alpha. |
| 供应商 | R&D Systems |
| 检测类型 | Solid Phase Sandwich ELISA |
| Entrez Gene IDs | 7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit) |
| 生物活性 | < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested. |
| 背景 | Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
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