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Rat VEGF Quantikine ELISA Kit (1 KT)

货号: RRV00 基本售价: 6791.4 元 规格: 1 Kit

产品信息

概述
货号RRV00
描述The Quantikine Rat VEGF Immunoassay is a 3.5 hour solid-phase ELISA designed to measure rat VEGF in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat VEGF and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat VEGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat VEGF.
别名MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor;
全称
Vascular Endothelial Growth Factor
反应种属Rat
目标/特异性Natural and recombinant rat VEGF. This assay cross-reacts 59% with Mouse VEGF120 (E.Coli-expressed), 73% with Mouse VEGF164 (Sf21-expressed), 14% with Canine VEGF164 (E.Coli-expressed), 26% with Human VEGF121 (Sf21-expressed), 31% with Human VEGF121 (E.Coli-expressed), 24% with Mouse VEGF162 (NS0-expressed), 0.2% with Human VEGF206 (E.Coli-expressed) and  5.2% with Human VEGF/PIGF.
性能
供应商R&D Systems
检测类型Solid Phase Sandwich ELISA
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Mesenchymal stem cells in corneal neovascularization: Comparison of different application routes
Mol Med Rep, 2016;0(0):.
Species: Rat
Sample Type: Tissue Homogenates

Optimal Route for Human Umbilical Cord Blood-Derived Mesenchymal Stem Cell Transplantation to Protect Against Neonatal Hyperoxic Lung Injury: Gene Expression Profiles and Histopathology.
Authors: Sung D, Chang Y, Ahn S, Sung S, Yoo H, Choi S, Kim S, Park W
PLoS ONE, 2015;10(8):e0135574.
Species: Rat
Sample Type: Tissue Homogenates

Leptin ameliorates ischemic necrosis of the femoral head in rats with obesity induced by a high-fat diet.
Authors: Zhou L, Jang K, Moon Y, Wagle S, Kim K, Lee K, Park B, Kim J
Sci Rep, 2015;5(0):9397.
Species: Rat
Sample Type: Plasma

Iron-hepcidin dysmetabolism, anemia and renal hypoxia, inflammation and fibrosis in the remnant kidney rat model.
Authors: Garrido P, Ribeiro S, Fernandes J, Vala H, Bronze-da-Rocha E, Rocha-Pereira P, Belo L, Costa E, Santos-Silva A, Reis F
PLoS ONE, 2015;10(4):e0124048.
Species: Rat
Sample Type: Serum

Systemic siRNA Nanoparticle-Based Drugs Combined with Radiofrequency Ablation for Cancer Therapy.
Authors: Ahmed M, Kumar G, Navarro G, Wang Y, Gourevitch S, Moussa M, Rozenblum N, Levchenko T, Galun E, Torchilin V, Goldberg S
PLoS ONE, 2015;10(7):e0128910.
Species: Rat
Sample Type: Tissue Homgenates

The effect of lithospermic acid, an antioxidant, on development of diabetic retinopathy in spontaneously obese diabetic rats.
Authors: Jin C, Yu S, Wang X, Woo S, Park H, Lee H, Choi S, Kim K, Kim J, Park K, Jang H, Lim S
PLoS ONE, 2014;9(6):e98232.
Species: Rat
Sample Type: ocular fluid

Antiangiogenic treatment diminishes renal injury and dysfunction via regulation of local AKT in early experimental diabetes.
Authors: Bai X, Li X, Tian J, Zhou Z
PLoS ONE, 2014;9(4):.
Species: Rat
Sample Type: Serum

Targeting Muller cell-derived VEGF164 to reduce intravitreal neovascularization in the rat model of retinopathy of prematurity.
Authors: Jiang Y, Wang H, Culp D, Yang Z, Fotheringham L, Flannery J, Hammond S, Kafri T, Hartnett M
Invest Ophthalmol Vis Sci, 2014;55(2):824-31.
Species: Rat
Sample Type: Serum

Antagonism of bradykinin B2 receptor prevents inflammatory responses in human endothelial cells by quenching the NF-kB pathway activation.
Authors: Terzuoli E, Meini S, Cucchi P, Catalani C, Cialdai C, Maggi C, Giachetti A, Ziche M, Donnini S
PLoS ONE, 2014;9(1):e84358.
Species: Rat
Sample Type: Synovial Fluid

Platelet rich plasma clot releasate preconditioning induced PI3K/AKT/NFkappaB signaling enhances survival and regenerative function of rat bone marrow mesenchymal stem cells in hostile microenvironments.
Authors: Peng Y, Huang S, Wu Y, Cheng B, Nie X, Liu H, Ma K, Zhou J, Gao D, Feng C, Yang S, Fu X
Stem Cells Dev, 2013;22(24):3236-51.
Species: Rat
Sample Type: cell culture supernate

Diosmin alleviates retinal edema by protecting the blood-retinal barrier and reducing retinal vascular permeability during ischemia/reperfusion injury.
Authors: Tong N, Zhang Z, Zhang W, Qiu Y, Gong Y, Yin L, Qiu Q, Wu X
PLoS ONE, 2013;8(4):e61794.

The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function.
Authors: Takehara, Yuji, Yabuuchi, Akiko, Ezoe, Kenji, Kuroda, Tomoko, Yamadera, Rie, Sano, Chiaki, Murata, Nana, Aida, Takuya, Nakama, Ken, Aono, Fumihito, Aoyama, Naoki, Kato, Keiich, Kato, Osamu
Lab Invest, 2013;93(2):181-93.

Angiopoietins/TIE2 system and VEGF are involved in ovarian function in a DHEA rat model of polycystic ovary syndrome.
Authors: Abramovich D, Irusta G, Bas D, Cataldi NI, Parborell F, Tesone M
Endocrinology, 2012;153(7):3446-56.
Species: Rat
Sample Type: Tissue Homogenates

Angiogenesis is present in experimental autoimmune encephalomyelitis and pro-angiogenic factors are increased in multiple sclerosis lesions.
Authors: Seabrook TJ, Littlewood-Evans A, Brinkmann V, Pollinger B, Schnell C, Hiestand PC
J Neuroinflammation, 2010;7(0):95.
Species: Rat
Sample Type: Tissue Homogenates

Toll-like receptor 2 mediates mesenchymal stem cell-associated myocardial recovery and VEGF production following acute ischemia-reperfusion injury.
Authors: Abarbanell AM, Wang Y, Herrmann JL
Am. J. Physiol. Heart Circ. Physiol., 2010;298(5):H1529-36.
Species: Mouse
Sample Type: Cell Culture Supernates

Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
Authors: Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S
Apoptosis, 2010;15(4):463-73.
Species: Rat
Sample Type: Cell Culture Supernates

Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1.
Authors: Ruiz de Almodovar C, Coulon C, Salin PA, Knevels E, Chounlamountri N, Poesen K, Hermans K, Lambrechts D, Van Geyte K, Dhondt J, Dresselaers T, Renaud J, Aragones J, Zacchigna S, Geudens I, Gall D, Stroobants S, Mutin M, Dassonville K, Storkebaum E, Jordan BF, Eriksson U, Moons L, DHooge R, Haigh JJ, Belin MF, Schiffmann S, Van Hecke P, Gallez B, Vinckier S, Chedotal A, Honnorat J, Thomasset N, Carmeliet P, Meissirel C
J. Neurosci., 2010;30(45):15052-66.
Species: Rat
Sample Type: Tissue Homogenates

Poly(Adenosine 5-diphosphate-ribose) polymerase inhibition counteracts multiple manifestations of experimental type 1 diabetic nephropathy.
Authors: Drel VR, Xu W, Zhang J, Pavlov IA, Shevalye H, Slusher B, Obrosova IG
Endocrinology, 2009;150(12):5273-83.
Species: Rat
Sample Type: Tissue Homogenates

The role of supplemental oxygen and JAK/STAT signaling in intravitreous neovascularization in a ROP rat model.
Authors: Byfield G, Budd S, Hartnett ME
Invest. Ophthalmol. Vis. Sci., 2009;50(7):3360-5.
Species: Rat
Sample Type: Tissue Homogenates

Phosphomannopentaose sulfate (PI-88) inhibits retinal leukostasis in diabetic rat.
Authors: Ma P, Luo Y, Zhu X, Ma H, Hu J, Tang S
Biochem. Biophys. Res. Commun., 2009;380(2):402-6.
Species: Rat
Sample Type: Tissue Homogenates

The effect of sustained delivery of vascular endothelial growth factor on angiogenesis in tissue-engineered intestine.
Authors: Rocha FG, Sundback CA, Krebs NJ, Leach JK, Mooney DJ, Ashley SW, Vacanti JP, Whang EE
Biomaterials, 2008;29(19):2884-90.
Species: Rat
Sample Type: Tissue Homogenates

Triamcinolone acetonide protects the rat retina from STZ-induced acute inflammation and early vascular leakage.
Authors: Kim YH, Choi MY, Kim YS, Park CH, Lee JH, Chung IY, Yoo JM, Choi WS, Cho GJ, Kang SS
Life Sci., 2007;81(14):1167-73.
Species: Rat
Sample Type: Tissue Homogenates

Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy.
Authors: Shimojo N, Jesmin S, Zaedi S, Otsuki T, Maeda S, Yamaguchi N, Aonuma K, Hattori Y, Miyauchi T
Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H474-81.
Species: Rat
Sample Type: Cell Culture Supernates

Nicotine-induced vascular endothelial growth factor release via the EGFR-ERK pathway in rat vascular smooth muscle cells.
Authors: Kanda Y, Watanabe Y
Life Sci., 2007;80(15):1409-14.
Species: Rat
Sample Type: Cell Culture Supernates

Bcl-2 engineered MSCs inhibited apoptosis and improved heart function.
Authors: Li W, Ma N, Ong LL, Nesselmann C, Klopsch C, Ladilov Y, Furlani D, Piechaczek C, Moebius JM, Lutzow K, Lendlein A, Stamm C, Li RK, Steinhoff G
Stem Cells, 2007;25(8):2118-27.
Species: Rat
Sample Type: Cell Culture Supernates

Insulin increases retinal hemorrhage in mild oxygen-induced retinopathy in the rat: inhibition by riluzole.
Authors: Yoo MH, Yoon YH, Chung H, Cho KS, Koh JY
Invest. Ophthalmol. Vis. Sci., 2007;48(12):5671-6.
Species: Rat
Sample Type: Tissue Homogenates

Adenosine A(2B) receptor mediates an increase on VEGF-A production in rat kidney glomeruli.
Authors: Valladares D, Quezada C, Montecinos P, Concha II, Yanez AJ, Sobrevia L, San Martin R
Biochem. Biophys. Res. Commun., 2007;366(1):180-5.
Species: Rat
Sample Type: Cell Culture Supernates

Inhibition of hypoxia-induced increase of blood-brain barrier permeability by YC-1 through the antagonism of HIF-1alpha accumulation and VEGF expression.
Authors: Yeh WL, Lu DY, Lin CJ, Liou HC, Fu WM
Mol. Pharmacol., 2007;72(2):440-9.
Species: Rat
Sample Type: Cell Culture Supernates

Improved vascular engraftment and function of autotransplanted pancreatic islets as a result of partial pancreatectomy in the mouse and rat.
Authors: Johansson M, Jansson L, Carlsson PO
Diabetologia, 2007;50(6):1257-66.
Species: Rat
Sample Type: Plasma

In the adult mesenchymal stem cell population, source gender is a biologically relevant aspect of protective power.
Authors: Crisostomo PR, Markel TA, Wang M, Lahm T, Lillemoe KD, Meldrum DR
Surgery, 2007;142(2):215-21.
Species: Rat
Sample Type: Tissue Homogenates

Combination therapy with the advanced glycation end product cross-link breaker, alagebrium, and angiotensin converting enzyme inhibitors in diabetes: synergy or redundancy?
Authors: Coughlan MT, Thallas-Bonke V, Pete J, Long DM, Gasser A, Tong DC, Arnstein M, Thorpe SR, Cooper ME, Forbes JM
Endocrinology, 2007;148(2):886-95.
Species: Rat
Sample Type: Urine

Enhancement of glucose transporter expression of brain endothelial cells by vascular endothelial growth factor derived from glioma exposed to hypoxia.
Authors: Yeh WL, Lin CJ, Fu WM
Mol. Pharmacol., 2007;73(1):170-7.
Species: Rat
Sample Type: Cell Culture Supernates

Exercise training improves aging-induced downregulation of VEGF angiogenic signaling cascade in hearts.
Authors: Iemitsu M, Maeda S, Jesmin S, Otsuki T, Miyauchi T
Am. J. Physiol. Heart Circ. Physiol., 2006;291(3):H1290-8.
Species: Rat
Sample Type: Tissue Homogenates

Salvage effect of the vascular endothelial growth factor on chemically induced acute severe liver injury in rats.
Authors: Namisaki T, Yoshiji H, Kojima H, Yoshii J, Ikenaka Y, Noguchi R, Sakurai S, Yanase K, Kitade M, Yamazaki M, Asada K, Uemura M, Nakamura M, Fukui H
J. Hepatol., 2006;44(3):568-75.
Species: Rat
Sample Type: Tissue Homogenates

Specific involvement of SRC family kinase activation in the pathogenesis of retinal neovascularization.
Authors: Werdich XQ, Penn JS
Invest. Ophthalmol. Vis. Sci., 2006;47(11):5047-56.
Species: Rat
Sample Type: Tissue Homogenates

Oxidative stress during peritoneal dialysis: implications in functional and structural changes in the membrane.
Authors: Noh H, Kim JS, Han KH, Lee GT, Song JS, Chung SH, Jeon JS, Ha H, Lee HB
Kidney Int., 2006;69(11):2022-8.
Species: Rat
Sample Type: Tissue Homogenates

Antagonism of vascular endothelial growth factor results in microvessel attrition and disorganization of wound tissue.
Authors: Gudehithlu KP, Ahmed N, Wu H, Litbarg NO, Garber SL, Arruda JA, Dunea G, Singh AK
J. Lab. Clin. Med., 2005;145(4):194-203.
Species: Rat
Sample Type: Granuloma Fluid

生物活性Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
背景VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.
运输条件Blue Ice
存放说明4℃
参考文献
  • Adipocytokines
  • Adult Neurogenesis
  • Airway Inflammation and Remodeling
  • ALS Related Molecules
  • BIObrief: Neuroprotective & Pathogenic Effects of VEGF Signaling
  • Cancer Biomarkers
  • Developmental Factors Implicated in Neurodegeneration
  • HIF Transcription Factors
  • HIF-1 & Vascular Development
  • ICC / IHC Images: VEGF
  • Inflammatory Mediators
  • Macrophage Activation Markers
  • Mini-review: Vascular Endothelial Growth Factor (VEGF)
  • Myeloid-derived Suppressor Cells (MDSC)
  • New and Improved TNF Superfamily Proteins 2015
  • Platelets
  • Preeclampsia
  • Proliferation Bioassay HUVECs
  • Regulation of VE-Cadherin and VEGF R2 by VEGF
  • Technical Notes: Serum Levels of Vascular Growth Factors During Pregnancy
  • VEGF & Amyotrophic Lateral Sclerosis
  • VEGF Family
  • VEGF Family Ligands
  • VEGF, Angiopoietin-2 and Tumor Vascularization