| 货号 | 94371C |
| 反应种属 | H M R Mk |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Total CDK5 Sandwich ELISA Kit detects endogenous levels of CDK5 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Cyclin-dependent kinases (CDKs) are serine/threonine kinases that are activated by cyclins and govern eukaryotic cell cycle progression. While CDK5 shares high sequence homology with its family members, it is thought mainly to function in postmitotic neurons to regulate the cytoarchitecture of these cells. Analogous to cyclins, the regulatory subunits p35 and p39 associate with and activate CDK5 despite the lack of sequence homology. CDK5 is ubiquitously expressed, with high levels of kinase activity detected primarily in the nervous system due to the narrow expression pattern of p35 and p39 in post-mitotic neurons. A large number of CDK5 substrates have been identified although no substrates have been specifically attributed to p35 or p39. Substrates of CDK5 include p35, PAK1, Src, β-catenin, tau, neurofilament-H, neurofilament-M, synapsin-1, APP, DARPP32, PP1-inhibitor, and Rb. p35 is rapidly degraded (T1/2 <20 min) by the ubiquitin-proteasome pathway (1). However, p35 stability increases as CDK5 kinase activity decreases, likely as a result of decreased phosphorylation of p35 at Thr138 by CDK5 (2). Proteolytic cleavage of p35 by calpain produces p25 upon neurotoxic insult, resulting in prolonged activation of CDK5 by p25. Research studies have shown accumulation of p25 in neurodegenerative diseases, such as Alzheimers disease and amyotrophic lateral sclerosis (ALS) (3,4). |
| 运输条件 | 0.75 |
| 存放说明 | 4C |
| 参考文献 | Dhavan, R. and Tsai, L.H. (2001) Nat Rev Mol Cell Biol 2, 749-59. Patrick, G.N. et al. (1998) J Biol Chem 273, 24057-64. Lee, M.S. et al. (2000) Nature 405, 360-4. Kusakawa, G. et al. (2000) J Biol Chem 275, 17166-72. |
Figure 1. Detection of CDK5 protein in HeLa, NIH/3T3, KNRK and COS-7 cells is examined by using the PathScan® Total CDK5 Sandwich ELISA Kit. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blot using CDK5 (1H3) Mouse mAb #12134 is shown in the bottom figure. | |
Figure 2. The relationship between protein concentration of lysates from HeLa cells and the absorbance at 450 nm as detected by the PathScan® Total CDK5 Sandwich ELISA Kit is shown. Unstarved HeLa cells (85% confluence) were harvested and then lysed. |
