| 货号 | 15078C |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Total FGF Receptor 4 Sandwich ELISA Kit detects endogenous levels of FGFR4 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5). |
| 存放说明 | 4C |
| 参考文献 | Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97. |
Figure 1. FGF receptor 4 protein is detected from multiple cell lines using the PathScan® Total FGF Receptor 4 Sandwich ELISA Kit #15078. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using FGF Receptor 4 (D3B12) XP® Rabbit mAb #8562 is shown in the bottom figure. | |
Figure 2. The relationship between protein concentration of lysates from Hep G2 cells and the absorbance at 450 nm as detected by the PathScan® Total FGF Receptor 4 Sandwich ELISA Kit #15078 is shown. Hep G2 cells (85% confluence) were harvested and then lysed. |
