PathScan® Total GFP Sandwich ELISA Kit
货号：
7878C 基本售价：
6346.0 元 规格：
1Kit
产品信息
概述| 货号 | 7878C |
| 描述 | The PathScan® Total GFP Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects exogenously expressed GFP. This kit is intended for use with cotransfected GFP as a convenient means of monitoring transfection efficiency. A GFP rabbit antibody has been coated onto the microwells. After incubation with cell lysates, GFP is captured by the coated antibody. Following extensive washing, a GFP mouse detection antibody is added to detect the captured GFP. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. The HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of GFP. |
| 应用 | ELISA |
| 目标/特异性 | CSTs PathScan® Total GFP Sandwich ELISA Kit #7878 detects GFP when exogenously expressed in cells. As shown in Figure 1, this kit readily detects GFP in transfected, but not in untransfected HEK293 cell lysate. This kit was developed for use with transfected full-length GFP and may not detect GFP fusion proteins. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
性能| 供应商 | CST |
| 背景 | The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea. GFP maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm (1). Among other applications, GFP is commonly cotransfected alongside a gene of interest as a means of monitoring transfection efficiency. |
| 存放说明 | 4C |
| 参考文献 | 1 . Miyawaki, A. et al. (2003) Curr Opin Chem Biol 7, 557-62.
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参考图片Figure 2. The relationship between the protein concentration of the lysate from untransfected and GFP-transfected HEK 293 cells and the absorbance at 450 nm is shown.图中所示为未转染组和GFP转染后的HEK293细胞提取物中蛋白浓度与450nm处吸光值之间的相关性分析。 |
Figure 1. Transfected GFP is detected by PathScan® Total GFP Sandwich ELISA Kit #7878. Lysates were prepared from control and GFP-transfected HEK293 cells and analyzed for GFP by PathScan® Total GFP Sandwich ELISA Kit #7878 or by Western blotting. Absorbance readings at 450 nm are shown in the top figure while the corresponding Western blot using GFP Antibody #2555 is shown in the bottom figure.用PathScan ® Total GFP Sandwich ELISA Kit #7878检测转染后的GFP。用对照和GFP转染后的HEK293细胞裂解后，收集提取物， 用PathScan ® Total GFP Sandwich ELISA Kit #7878 或者western blot分析GFP。如上图所示在450nm处读取吸光度，下图为用抗GFP抗体#2555相对应的western blot。 |
