| 货号 | 7149C |
| 描述 | The PathScan® Phospho-Stat3 (Tyr705) Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-Stat3 (Tyr705) protein with a chemiluminescent readout. Chemiluminescent ELISAs often have a wider dynamic range and higher sensitivity than conventional chromogenic detection. This chemiluminescent ELISA, which is offered in low volume microplates, shows increased signal and sensitivity while using smaller sample volume. A Stat3 antibody has been coated on the microwells. After incubation with cell lysates, phospho and nonphospho-Stat3 proteins are captured by the coated antibody. Following extensive washing, a phospho-Stat3 mouse mAb is added to detect the captured phospho-Stat3 (Tyr705) protein. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. Chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of phospho-Stat3 (Tyr705) protein. |
| 反应种属 | Human, Mouse |
| 目标/特异性 | PathScan® Phospho-Stat3 (Tyr705) Chemiluminescent Sandwich ELISA Kit detects endogenous levels of phospho-Stat3 (Tyr705) in human and mouse cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | 4C |
| 参考文献 | Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85. Bromberg, J.F. et al. (1999) Cell 98, 295-303. Darnell, J.E. et al. (1994) Science 264, 1415-21. Ihle, J.N. (1995) Nature 377, 591-4. Wen, Z. et al. (1995) Cell 82, 241-50. Yokogami, K. et al. (2000) Curr Biol 10, 47-50. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94. |
Relationship between protein concentration of lysates from untreated and IFN-α treated HeLa cells and immediate light generation with chemiluminescent substrate is shown. Cells (75% confluence) were treated with IFN-α (100 ng/ml) and lysed after incubation at 37ºC for 10 minutes. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.图示为未经处理和经IFN-α处理的HeLa细胞的裂解液蛋白浓度与直接加入化学发光底物产生光关系。HeLa细胞(75% confluence)在37ºC 条件下用IFN-α (100 ng/ml)处理10Min,并裂解。插入到对应灰色区域中的图表显示了高敏感度和在低蛋白浓度范围下的线性反应关系。 |
