| 货号 | 12384C |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Total Caveolin-1 Sandwich ELISA Kit recognizes endogenous levels of Caveolin-1 protein as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homo-oligomers that interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins interact with multiple signaling molecules such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6). |
| 存放说明 | 4C |
| 参考文献 | Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22. Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304. Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986. Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103. Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75. Schlegel, A. et al. (2001) J Biol Chem 276, 4398-408. |
Figure 1. Caveolin-1 protein from human (HeLa), monkey (COS-7), Hamster (CHO), mouse (NIH/3T3), and rat (C6) cells was detected using PathScan® Total Caveolin-1 Sandwich ELISA Kit. However, this kit has a low detection level for Caveolin-1 in C2C12 cells and has no detection in PC-12 cells. The absorbance readings at 450 nm are shown in the top figure, while corresponding western blots using Caveolin-1 (D46G3) XP® Rabbit mAb #3267 are shown in the bottom figure. 使用PathScan® Total Caveolin-1 Sandwich ELISA检测来自人(HeLa),猴(COS-7),仓鼠(CHO),小鼠(NIH/3T3)和大鼠(C6)的Caveolin-1。但是该试剂盒很难检测到C2C12细胞中的Caveolin-1，并且无法检测到PC-12细胞中的Caveolin-1.450nm处的吸光值如上图所示，同时使用Caveolin-1 (D46G3) XP® Rabbit mAb #3267进行western blot试验的数据如下图。 | |
Figure 2. The relationship between protein concentration of lysates from HeLa cells and the absorbance at 450 nm is shown. HeLa cells (85% confluence) were harvested and then lysed. HeLa细胞裂解液中蛋白浓度和450nm吸光值的关系如图所示。HeLa（85%接触）的细胞被收集随后被裂解。 |
