PathScan® Total Stat6 Sandwich ELISA Kit【科瑞奥博】

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PathScan® Total Stat6 Sandwich ELISA Kit

货号： 7267C 基本售价： 6346.0 元规格： 1Kit

产品信息

概述
货号	7267C
描述	The PathScan® Total Stat6 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Stat6 protein. A Stat6 Mouse mAb has been coated onto the microwells. After incubation with cell lysates, Stat6 protein is captured by the coated antibody. Following extensive washing, a Stat6 Rabbit Dectection Antibody is added to detect captured Stat6 protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of Stat6 protein.   Antibodies in kit are custom formulations specific to kit.
反应种属	Human/Mouse
应用	ELISA
目标/特异性	PathScan® Total Stat6 Sandwich ELISA Kit detects endogenous levels of Stat6 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

性能
供应商	CST
背景	Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cells, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).
存放说明	4C
参考文献	Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872. Hou, J. et al. (1994) Science 265, 1701-1706. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343. Takeda, K. et al. (1996) Nature 380, 627-630. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

参考图片

Figure 2. Relationship between protein concentration of lysates from ACHN cells, untreated or treated with Human Interleukin-4 (hIL-4) #8919, and the absorbance at 450 nm is shown. Cells (80% confluence) were treated with hIL-4 (100 ng/ml) and lysed after incubation at 37ºC for 15-20 min.图2.图示为未经处理和经过hIL-4 #8919处理的ACHN细胞裂解液蛋白浓度与试剂盒实验OD450读数之间的关系。ACHN细胞(80-90% confluent)在37ºC条件下经过hIL-4 (100 ng/ml)处理 15-20分钟然后裂解。

Figure 1. Treatment of ACHN cells with Human Interleukin-4 (hIL-4) #8919 stimulates phosphorylation of Stat6 at Tyr641, as detected by PathScan® Phospho-Stat6 (Tyr641) Sandwich ELISA Kit #7275, but does not affect levels of total Stat6 protein detected by PathScan® Total Stat6 Sandwich ELISA Kit #7267. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Stat6 Antibody #9362 (left panel) or Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb #9364 (right panel) are shown in the bottom figure.图1.ACHN细胞经过hIL-4 #8919刺激后产生Tyr641位点磷酸化的Stat6 然后经过PathScan® Phospho-Stat6 (Tyr641) Sandwich ELISA Kit #7275检测,同时经过PathScan® Total Stat6 Sandwich ELISA Kit #7267检测发现处理后并未影响Stat6蛋白的总量。OD 450 的读数在图片的最上面显示, 同时对应的Western免疫印迹在下面图片显示，所用抗体为Stat6 Antibody #9362 (左图) 或 Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb #9364 (右图)。