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货号： bs-15124R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-15124R
- 英文名称
- C21orf33
- 中文名称
- 21号染色体开放阅读框33抗体
- 别 名
- Chromosome 21 open reading frame 33; D21S2048E; ES 1; ES1; Es1 protein; ES1 protein homolog mitochondrial; GT335; HES 1; HES1; Human HES1 protein homolog to E.coli and zebrafish ES1 protein; Keio novel protein I; KNP I; KNPH; KNPI; Protein GT335; Protein KNP I; ES1_HUMAN.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 免疫学 线粒体
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 28kDa
- 细胞定位
- 细胞浆 线粒体
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human C21orf33:101-200/268
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
KNP-I is a ubiquitously expressed mitochondrial protein that belongs to the ES1 family of proteins. It is a highly conserved protein with homologs identified in various species. This conserved nature suggests an important biological role for the KNP-I protein. The gene that encodes KNP-I (C21orf33) is located on human chromosome 21 in close proximity to a region (D21S25) associated with many genetic diseases. Down syndrome (DS), caused by an extra copy of chromosome 21, is the most common manifestation of trisomic chromosomes. It is likely that the overproduction of the C21orf33 gene product plays a role in the pathology of DS, while its chromosomal location suggests its likely involvement in D21S25 related diseases. While expressed in most tissue, highest expression of KNP-I is found in heart and muscle
Subcellular Location:
Mitochondrion (Potential).
Tissue Specificity:
Ubiquitous, but strongly expressed in heartand skeletal muscle.
Similarity:
Belongs to the ES1 family.
SWISS:
P30042
Gene ID:
8209
Database links:Entrez Gene: 8209Human
Omim: 601659Human
SwissProt: P30042Human
Unigene: 413482Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-C21orf33 (bs-15124R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Tissue/cell: Rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-C21orf33 Polyclonal Antibody, Unconjugated(bs-15124R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1ug in 100 uL1X PBS containing 0.5% BSA(green).