| 货号 | 18157C |
| 反应种属 | Human\Mouse\Moneky |
| 目标/特异性 | PathScan® Total Bmi1 Sandwich ELISA Kit detects endogenous levels of Bmi1protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 使用方法 | ELISA |
| 供应商 | CST |
| 背景 | The polycomb group (PcG) of proteins contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyl-transferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (6). Bmi1 is a component of the PRC1 complex, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit (7). Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and is required for maintenance of adult hematopoietic and neural stem cells (3,4,8-10). |
| 存放说明 | 4C |
| 参考文献 | Boyer, L.A. et al. (2006) Nature 441, 349-53. Lee, T.I. et al. (2006) Cell 125, 301-13. Park, I.K. et al. (2003) Nature 423, 302-5. Molofsky, A.V. et al. (2003) Nature 425, 962-7. Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67. Wang, H. et al. (2004) Nature 431, 873-8. Cao, R. et al. (2005) Mol Cell 20, 845-54. Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7. Jacobs, J.J. et al. (1999) Nature 397, 164-8. Jacobs, J.J. et al. (1999) Genes Dev 13, 2678-90. |
Figure 1. Detection of Bmi1 protein in HeLa, NIH/3T3, and COS-7 cells is examined by using the PathScan® Total Bmi1 Sandwich ELISA Kit. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blot using Bmi1 (D42B3) Rabbit mAb #5856 is shown in the bottom figure. | |
Figure 2. The relationship between protein concentration of lysates from HeLa cells and the absorbance at 450 nm as detected by the PathScan® Total Bmi1 Sandwich ELISA Kit is shown. Unstarved HeLa cells (85% confluence) were harvested and then lysed. |
