| 货号 | 7996C |
| 描述 | The PathScan® Total Acetyl-CoA Carboxylase (ACC) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of ACC. An ACC mouse antibody has been coated onto the microwells. After incubation with cell lysates, ACC (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, an ACC rabbit detection antibody is added to detect captured ACC protein. Anti-rabbit IgG, HRP-linked Antibody #7074 is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total ACC. |
| 反应种属 | Human/Mouse |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Total Acetyl-CoA Carboxylase Sandwich ELISA Kit #7996 detects endogenous levels of total ACC protein (see Figure 1). The kit sensitivity is shown in Figure 2. |
| 供应商 | CST |
| 背景 | Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα (ACC1) is the predominant isoform found in liver, adipocytes, and mammary gland, while the 280 kDa ACCβ (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4). |
| 存放说明 | 4C and -20C |
| 参考文献 | Castle, J.C. et al. (2009) PLoS One 4, e4369. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86. Ha, J. et al. (1994) J Biol Chem 269, 22162-8. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50. |
Figure 1. Treatment of Hep G2 cells with H2O2 stimulates phosphorylation of ACC at Ser79, detected by the PathScan® Phospho-ACC (Ser79) Sandwich ELISA Kit #7986, but does not affect the levels of total ACC detected by PathScan® Total ACC Sandwich ELISA Kit #7996. Hep G2 cells (80-90% confluent) were treated 10 mM hydrogen peroxide for 10 minutes and lysed with #7018. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (left panel) or Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661 (right panel) are shown in the bottom figure. 图1，用过氧化氢处理Hep G2细胞会增加ACC在Ser79位点的磷酸化水平，检测试剂盒为PathScan® Phospho-ACC (Ser79) Sandwich ELISA Kit #7986。但不影响ACC总蛋白水平，检测试剂盒为PathScan® Total ACC Sandwich ELISA Kit #7996。Hep G2用10 mM过氧化氢处理10分钟，然后用#7018裂解。上图所示为450nm处的吸光度值，下图所示为相应的Western blot检测，所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗 #3676(左)，Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661(右)。 | |
Figure 2. The relationship between the protein concentration of lysates from untreated and H2O2-treated Hep G2 cells and the absorbance at 450 nm using the PathScan® Total ACC Sandwich ELISA Kit #7996 is shown. 图2，Hep G2细胞裂解液中蛋白浓度与450钠米处吸光度的关系图，所用细胞未处理或用过氧化氢处理，所用试剂盒为PathScan® Total ACC Sandwich ELISA Kit #7996 |
