| 货号 | 7169C |
| 描述 | CSTs PathScan® Total Survivin Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total survivin protein. A Survivin Mouse mAb* has been coated onto the microwells. After incubation with cell lysates, both phospho and nonphospho survivin proteins are captured by the coated antibody. Following extensive washing, Survivin Rabbit Detection Antibody* is added to detect the captured survivin protein. Anti-rabbit IgG, HRP-linked Antibody #7074* is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for this developed color is proportional to the quantity of total survivin protein. * Antibodies in kit are custom formulations specific to kit. |
| 反应种属 | Human/Monkey |
| 应用 | ELISA |
| 目标/特异性 | CSTs PathScan® Total Survivin Sandwich ELISA Kit #7169 detects endogenous levels of total survivin protein. As shown in Figure 1, survivin protein from human (HeLa, H526 and MCF-7) and monkey (COS), is detected by this kit. In Figure 3, Western analysis of protein captured in microwells coated with the survivin capture antibody shows a major band corresponding to the survivin protein (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6). |
| 存放说明 | 4C |
| 参考文献 | Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200. Li, F. et al. (1998) Nature 396, 580-584. Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466. OConnor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107. Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809. Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640. |
Figure 1: Survivin protein from human cells (HeLa, H526 and MCF-7) and monkey cells (COS) can be detected by PathScan® Total Survivin Sandwich ELISA Kit #7169. However, this kit cannot detect the survivin protein from other species, such as mink (MV1LU), rat (C6), mouse (BAF3) and hamster (CHO) cell lines. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blot using Survivin (6E4) Mouse mAb #2802, is shown in the bottom figure. 图1：PathScan® Total Survivin Sandwich ELISA Kit #7169检测人细胞(HeLa、H526和MCF-7)和猴细胞(COS)裂解液中Survivin蛋白的含量。但是该试剂盒不能检测其它物种细胞系如水貂（MV1LU）、大鼠（C6）、小鼠（BAF3）和仓鼠（CHO）中相应的蛋白。450nm吸光值见顶图，相应的免疫印迹检测结果见底图，使用抗体为Survivin (6E4) Mouse mAb 鼠单抗#2802。 | |
Figure 2: The relationship between protein concentration of lysates from HeLa cells and the absorbance at 450 nm is shown. HeLa cells (80% confluence) were harvested and then lysed. 图2：Hela细胞裂解液蛋白浓度和试剂盒测得的450nm光密度读数之间的线性关系。收集并HeLa细胞（80％丰度）。 |
