货号 | 14119C |
反应种属 | Human |
应用 | ELISA |
目标/特异性 | PathScan® Total PSA/KLK3 Sandwich ELISA Kit detects endogenous levels of PSA/KLK3 protein as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
供应商 | CST |
背景 | Kallikrein 3 (KLK3), also known as Prostate Specific Antigen (PSA), is a member of the glandular kallikrein subfamily of serine proteases (1). It is produced by prostate epithelial cells and is secreted into prostatic ducts. Upon cleavage of 7 amino-terminal amino acids (2), it is activated to liquefy semen in the seminal coagulum. Although PSA/KLK3 is produced in healthy individuals, investigators have found abnormally high levels in the blood of men with advanced prostate cancer (2,3). |
存放说明 | 4C |
参考文献 | Watt, K.W. et al. (1986) Proc Natl Acad Sci U S A 83, 3166-70. Balk, S.P. et al. (2003) J Clin Oncol 21, 383-91. Thompson, I.M. et al. (2004) N Engl J Med 350, 2239-46. |
Figure 1. PSA/KLK3 protein is expressed in LNCaP cells but absent in other cell lines, as detected using the PathScan® Total PSA/KLK3 Sandwich ELISA Kit #14119. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using PSA/KLK3 (D6B1) XP® Rabbit mAb #5365 is shown in the bottom figure. | |
Figure 2. The relationship between protein concentration of lysates from LNCaP cells and the absorbance at 450 nm as detected by the PathScan® Total PSA/KLK3 Sandwich ELISA Kit is shown. LNCaP cells (85% confluence) were harvested and lysed. |