| 货号 | 13125C |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Phospho-HER4/ErbB4 (panTyr) Sandwich ELISA Kit #13125 detects endogenous levels of tyrosine-phosphorylated HER4/ErbB4 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Research studies have implicated the HER/ErbB receptor tyrosine kinase family in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in research studies suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3). |
| 存放说明 | 4C |
| 参考文献 | Holbro, T. and Hynes, N.E. (2004) Annu Rev Pharmacol Toxicol 44, 195-217. Roskoski, R. (2004) Biochem Biophys Res Commun 319, 1-11. Carpenter, G. (2003) Exp Cell Res 284, 66-77. |
Figure 1: Treatment of T-47D cells with Human Neuregulin-1 (hNRG-1) #5218 stimulates tyrosine phosphorylation of HER4/ErbB4 protein, as detected by PathScan® Phospho-HER4/ErbB4 (panTyr) Sandwich ELISA Kit #13125, but does not affect the level of total HER4/ErbB4 detected by PathScan® Total HER4/ErbB4 Sandwich ELISA Kit #13010. The absorbance readings at 450 nm are shown in the top figure while corresponding western blots using HER4/ErbB4 (111B2) Rabbit mAb #4795 (left panel) and Phospho-HER4/ErbB4 (Tyr984) Antibody #3790 (right panel) are shown in the bottom figure. | |
Figure 2: The relationship between protein concentration of lysates from untreated and hNRG-1-treated T-47D cells and the absorbance at 450 nm as detected by the PathScan® Phospho-HER4/ErbB4 (panTyr) Sandwich ELISA Kit is shown. Starved T-47D cells (85% confluence) were treated with Human Neuregulin-1 (hNRG-1) #5218 (10 ng/ml) for 2-5 min at 37°C and then lysed. |
