| 货号 | 12909C |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Phospho-FGF Receptor 1 (panTyr) Sandwich ELISA Kit detects endogenous levels of tyrosine-phosphorylated FGFR1 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5). |
| 存放说明 | 4C |
| 参考文献 | Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97. Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8. Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89. Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78. Larsson, H. et al. (1999) J Biol Chem 274, 25726-34. |
Figure 1: Treatment of A-204 cells with Human Basic Fibroblast Growth Factor (hFGF basic/FGF2) #8910 stimulates tyrosine phosphorylation of FGFR1, as detected by the PathScan® Phospho-FGF Receptor 1 (panTyr) Sandwich ELISA Kit, but does not affect the level of total FGFR1 detected by the PathScan® Total FGF Receptor 1 Sandwich ELISA Kit #12828. Absorbance at 450 nm is shown in the top figure while corresponding western blots using FGF Receptor 1 (D8E4) XP® Rabbit mAb #9740 (left) and Phospho-FGF Receptor (Tyr653/654) Antibody #3471 (right) are shown in the bottom figure. | |
Figure 2: The relationship between protein concentration of lysates from untreated and hFGF basic-treated A-204 cells and the absorbance at 450 nm as detected by PathScan® Phospho-FGF Receptor 1 (panTyr) Sandwich ELISA Kit is shown. Unstarved A-204 cells (85% confluence) were treated with Human Basic Fibroblast Growth Factor (hFGF basic/FGF2) #8910 (100 ng/ml) for 2-5 min at 37°C and then lysed. |
